Anti-MTERFD2抗体(ab121910)
Key features and details
- Rabbit polyclonal to MTERFD2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MTERFD2抗体 -
描述
兔多克隆抗体to MTERFD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to N terminal amino acids 23-152 of Human MTERFD2.
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阳性对照
- MTERFD2 over-expression HEK293T cell lysate; Human skeletal muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121910于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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序列相似性
Belongs to the mTERF family. - Information by UniProt
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数据库链接
- Entrez Gene: 130916 Human
- SwissProt: Q7Z6M4 Human
- Unigene: 159556 Human
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别名
- FLJ16261 antibody
- HSPC096 antibody
- MGC61716 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MTERFD2 antibody (ab121910)Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121910 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-MTERFD2 antibody (ab121910)All lanes : Anti-MTERFD2 antibody (ab121910) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : MTERFD2 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERFD2 antibody (ab121910)ab121910, at 1/60 dilution, staining MTERFD2 in paraffin embedded Human skeletal muscle tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121910 被引用在 1 文献中.
- Pan J et al. Integration of IgA and IgG Autoantigens Improves Performance of Biomarker Panels for Early Diagnosis of Lung Cancer. Mol Cell Proteomics 19:490-500 (2020). PubMed: 31924693