Anti-MTCO2抗体[12C4F12] (ab110258)
![Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.cn/ps/products/110/ab110258/Images/ab110258-123443-anti-mtco2-antibody-12c4f12-western-blot.jpg "Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)")
Key features and details
- Mouse monoclonal [12C4F12] to MTCO2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-MTCO2抗体[12C4F12]
参阅全部 MTCO2 一抗 -
描述
小鼠单克隆抗体[12C4F12] to MTCO2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- WB: Mitochondrial lysate from human heart tissue. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE. -
克隆
单克隆 -
克隆编号
12C4F12 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (11) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
|
| ICC/IF | (2) |
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal) |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
疾病相关
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
序列相似性
Belongs to the cytochrome c oxidase subunit 2 family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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别名
- COII antibody
- COX 2 antibody
- COX II antibody
see all
图片
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Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Mitochondrial lysate from human heart tissue at 5 µg
Predicted band size: 26 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.cn/ps/products/110/ab110258/Images/ab110258-123442-anti-mtco2-antibody-12c4f12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
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![Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.cn/ps/products/110/ab110258/Images/ab110258-123441-anti-mtco2-antibody-12c4f12-flow-cytometry.jpg)
Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (233)
ab110258 被引用在 233 文献中.
- Hayashida R et al. Elucidation of ubiquitin-conjugating enzymes that interact with RBR-type ubiquitin ligases using a liquid-liquid phase separation-based method. J Biol Chem 299:102822 (2023). PubMed: 36563856
- Cafournet C et al. Novel ELAC2 Mutations in Individuals Presenting with Variably Severe Neurological Disease in the Presence or Absence of Cardiomyopathy. Life (Basel) 13:N/A (2023). PubMed: 36836802
- Guo Q et al. Organoid-derived human retinal progenitor cells promote early dedifferentiation of Müller glia in Royal College of Surgeons rats. Int J Ophthalmol 16:483-498 (2023). PubMed: 37077494
- Skopkova M et al. ATAD3A-related pontocerebellar hypoplasia: new patients and insights into phenotypic variability. Orphanet J Rare Dis 18:92 (2023). PubMed: 37095554
- Yue M et al. Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production. EMBO J 42:e112542 (2023). PubMed: 37218505