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重组

重组Anti-MTCO1抗体[1D6E1A8] (ab14705)

Reviews (42)Q&A (27)References (522)
Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1D6E1A8] to MTCO1
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

概述

  • 产品名称

    Anti-MTCO1抗体[1D6E1A8]
    参阅全部 MTCO1 一抗

  • 描述

    小鼠单克隆抗体[1D6E1A8] to MTCO1

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, IHC-P, WB, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Goat, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset

  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, MCF7, C6, PC-12, Neuro-2a, RAW 264.7 whole cell lysates. Human skeletal, Rat heart and Rat liver tissue lysates. IHC-P: Human kidney, Mouse colon, and Rat colon tissue. ICC: Human cervical adenocarcinoma, Mouse neuroblastoma neuroblast, and rat glial tumor cells. Flow Cyt: HeLa, Neuro-2a, and C6 cells.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 24th May 2023.


    Western blot protocol advice:
    For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal.

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab14705于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (2)
1/100.
IHC-P (13)
1/5000.
WB (18)
1/1000.
Flow Cyt (1)
1/500.
说明
ICC
1/100.
IHC-P
1/5000.
WB
1/1000.
Flow Cyt
1/500.

靶标

  • 功能

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

  • 通路

    Energy metabolism; oxidative phosphorylation.

  • 疾病相关

    Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].

  • 序列相似性

    Belongs to the heme-copper respiratory oxidase family.

  • 细胞定位

    Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • COI antibody
    • COX I antibody
    • COX1 antibody
    • COX1_HUMAN antibody
    • COXI antibody
    • Cytochrome c oxidase polypeptide I antibody
    • Cytochrome c oxidase subunit 1 antibody
    • Cytochrome C Oxidase subunit I antibody
    • Mitochondrially encoded cytochrome c oxidase I antibody
    • MT CO1 antibody
    • MT-CO1 antibody
    • MTCO 1 antibody
    • MTCO1 antibody
    see all

图片

  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) non-mitochondrial fraction
    Lane 2 : HeLa mitochondrial fraction
    Lane 3 : MCF7(human breast adenocarcinoma epithelial cell) non-mitochondrial fraction
    Lane 4 : MCF7 mitochondrial fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 5 : Human skeletal muscle tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human cervical adenocarcinoma epithelial cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Human cervical adenocarcinoma epithelial cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse neuroblastoma neuroblast cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Mouse neuroblastoma neuroblast cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat glial tumor cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Rat glial tumor cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of Human HeLa cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of Neuro-2a cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of C6 cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 3b doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    This image was generated using a previous batch manufactured using hybridoma production method.

    250 μm human cerebellar sections from control individuals and a patient with mitochondrial disease underwent passive clearing at 37 °C for 2 or 4 weeks.

    The quality of immunofluorescent staining is determined by duration of passive clearing; 2 weeks of passive clearing produced minimal labelling of the white matter in the granule cell layer (NF-H; green; 488 nm and MBP; red, 546 nm) with an absence of labelling of mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm; Extending passive clearing to 4 weeks improved the quality of stain with identifiable Purkinje cells and their axons (NF-H, green; 488 nm) and their myelin sheaths (MBP; red, 546 nm) and mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm.

文献 (522)

发表研究结果有使用 ab14705？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab14705 被引用在 522 文献中.

  • Chen CL  et al. SDHAF2 facilitates mitochondrial respiration through stabilizing succinate dehydrogenase and cytochrome c oxidase assemblies. Mitochondrion 79:101952 (2024). PubMed: 39237068
  • Kane MS  et al. The interplay between sex, time of day, fasting status, and their impact on cardiac mitochondrial structure, function, and dynamics. Sci Rep 13:21638 (2023). PubMed: 38062139
  • Rimoldi M  et al. Prominent muscle involvement in a familial form of mitochondrial disease due to a COA8 variant. Front Genet 14:1278572 (2023). PubMed: 38098475
  • Benegiamo G  et al. The genetic background shapes the susceptibility to mitochondrial dysfunction and NASH progression. J Exp Med 220:N/A (2023). PubMed: 36787127
  • Malik N  et al. Dysregulation of Mitochondrial Translation Caused by CBFB Deficiency Cooperates with Mutant PIK3CA and Is a Vulnerability in Breast Cancer. Cancer Res 83:1280-1298 (2023). PubMed: 36799863
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry abreview for Anti-MTCO1 [1D6E1A8] antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
Specification
Mouse Embryonic Fibroblasts
Permeabilization
Yes - 0.5% Triton-X
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 26 2014

Immunocytochemistry abreview for Anti-MTCO1 [1D6E1A8] antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cultured Cells (H460)
Specification
H460
Permeabilization
Yes - 0.5% Triton-X
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 26 2014

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