Anti-MTAP抗体[2G4] (ab55517)
![Western blot - Anti-MTAP antibody [2G4] (ab55517)](https://www.abcam.cn/ps/products/55/ab55517/Images/ab55517-68816-anti-mtap-antibody-western-blot.jpg "Western blot - Anti-MTAP antibody [2G4] (ab55517)")
Key features and details
- Mouse monoclonal [2G4] to MTAP
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MTAP抗体[2G4]
参阅全部 MTAP 一抗 -
描述
小鼠单克隆抗体[2G4] to MTAP -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human MTAP. Immunogen based on GenBank: AAH18625.1.
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常规说明
This product was changed from ascites to tissue culture supernatant on 30 April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
2G4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55517于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the PNP/MTAP phosphorylase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4507 Human
- Omim: 156540 Human
- SwissProt: Q13126 Human
- Unigene: 193268 Human
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别名
- 5' methylthioadenosine phosphorylase antibody
- 5''-methylthioadenosine phosphorylase antibody
- BDMF antibody
see all
图片
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Western blot - Anti-MTAP antibody [2G4] (ab55517)
MTAP antibody (ab55517) at 1 μg/lane + HeLa cell lysate at 25 μg/lane.
This image was genrated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [2G4] (ab55517)](https://www.abcam.cn/ps/products/55/ab55517/Images/ab55517-68815-anti-mtap-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [2G4] (ab55517)ab55517 at 10 μg/ml staining MTAP in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was genrated using the ascites version of the product.
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![Flow Cytometry - Anti-MTAP antibody [2G4] (ab55517)](https://www.abcam.cn/ps/products/55/ab55517/Images/ab55517-68814-anti-mtap-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-MTAP antibody [2G4] (ab55517)Overlay histogram showing HeLa cells stained with ab55517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55517, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This image was genrated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab55517 被引用在 3 文献中.
- Limm K et al. Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma. PLoS One 11:e0160348 (2016). WB . PubMed: 27479139
- Woollard WJ et al. Independent Loss of Methylthioadenosine Phosphorylase (MTAP) in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol 136:1238-46 (2016). PubMed: 26872600
- Czech B et al. Expression and function of methylthioadenosine phosphorylase in chronic liver disease. PLoS One 8:e80703 (2013). WB, IHC-P ; Human . PubMed: 24324622
