重组Anti-MTA3抗体[EPR12777(B)] (ab176346)
![Western blot - Anti-MTA3 antibody [EPR12777(B)] (ab176346)](https://www.abcam.cn/ps/products/176/ab176346/Images/ab176346-203773-anti-mta3-antibody-epr12777b-western-blot.png "Western blot - Anti-MTA3 antibody [EPR12777(B)] (ab176346)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12777(B)] to MTA3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MTA3抗体[EPR12777(B)]
参阅全部 MTA3 一抗 -
描述
兔单克隆抗体[EPR12777(B)] to MTA3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human MTA3 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BTC8 -
阳性对照
- Permeabilized Ramos cells; T47-D, HeLa, MCF-7 and Ramos cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12777(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. -
组织特异性
No expression in nonepithelial cells. -
序列相似性
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 57504 Human
- Entrez Gene: 116871 Mouse
- Entrez Gene: 298763 Rat
- Omim: 609050 Human
- SwissProt: Q9BTC8 Human
- SwissProt: Q924K8 Mouse
- Unigene: 435413 Human
- Unigene: 277668 Mouse
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别名
- 1110002J22Rik antibody
- fj99h01 antibody
- KIAA1266 antibody
see all
图片
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Western blot - Anti-MTA3 antibody [EPR12777(B)] (ab176346)All lanes : Anti-MTA3 antibody [EPR12777(B)] (ab176346) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
![Flow Cytometry (Intracellular) - Anti-MTA3 antibody [EPR12777(B)] (ab176346)](https://www.abcam.cn/ps/products/176/ab176346/Images/ab176346-203774-anti-mta3-antibody-epr12777b-flow-cytometry.png)
Flow Cytometry (Intracellular) - Anti-MTA3 antibody [EPR12777(B)] (ab176346)Intracellular flow cytometric analysis of permeabilized Ramos cells labeling MTA3with ab176346 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
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![Anti-MTA3 antibody [EPR12777(B)] (ab176346)](https://www.abcam.cn/ps/products/176/ab176346/Images/ab176346-3-benefits-of-recombinant-antibodies.png)
Anti-MTA3 antibody [EPR12777(B)] (ab176346)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab176346 被引用在 5 文献中.
- Žemaitis K et al. The stem cell-supporting small molecule UM171 triggers Cul3-KBTBD4-mediated degradation of ELM2 domain-harboring proteins. J Biol Chem 299:104662 (2023). PubMed: 36997086
- Xiaomeng F et al. Treatment with ß-elemene combined with paclitaxel inhibits growth, migration, and invasion and induces apoptosis of ovarian cancer cells by activation of STAT-NF-?B pathway. Braz J Med Biol Res 53:e8885 (2020). PubMed: 32401925
- Li Q et al. High glucose promotes hepatic fibrosis via miR-32/MTA3-mediated epithelial-to-mesenchymal transition. Mol Med Rep 19:3190-3200 (2019). PubMed: 30816482
- Wang Z et al. ß-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells. Oncol Lett 16:2143-2150 (2018). PubMed: 30008912
- Wei FZ et al. Epigenetic regulation of autophagy by the methyltransferase EZH2 through an MTOR-dependent pathway. Autophagy 11:2309-22 (2015). PubMed: 26735435