Anti-MTA2/PID抗体(ab8106)
Key features and details
- Rabbit polyclonal to MTA2/PID
- Suitable for: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MTA2/PID抗体
参阅全部 MTA2/PID 一抗 -
描述
兔多克隆抗体to MTA2/PID -
宿主
Rabbit -
特异性
No cross-reactivity to MTA1. -
经测试应用
适用于: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 18413351
|
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| ELISA |
Use at an assay dependent concentration.
|
|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| IP | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 18413351 |
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9219 Human
- Entrez Gene: 23942 Mouse
- Entrez Gene: 361724 Rat
- Omim: 603947 Human
- SwissProt: O94776 Human
- SwissProt: Q9R190 Mouse
- Unigene: 173043 Human
- Unigene: 25339 Mouse
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别名
- DKFZp686F2281 antibody
- Mata1l1 antibody
- Metastasis associated 1 family member 2 antibody
see all
图片
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Western blot - Anti-MTA2/PID antibody (ab8106)All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (ab8106)ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
实验方案
数据表及文件
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Datasheet download
文献 (31)
ab8106 被引用在 31 文献中.
- Wang W et al. Zinc-finger protein CXXC5 promotes breast carcinogenesis by regulating the TSC1/mTOR signaling pathway. J Biol Chem 299:102812 (2023). PubMed: 36539038
- Deng P et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer. J Clin Invest 132:N/A (2022). PubMed: 36201246
- Apipongrat D et al. Serum proteomic profiling reveals MTA2 and AGO2 as potential prognostic biomarkers associated with disease activity and adverse outcomes in multiple myeloma. PLoS One 17:e0278464 (2022). PubMed: 36454786
- Vinjamur DS et al. ZNF410 represses fetal globin by singular control of CHD4. Nat Genet 53:719-728 (2021). PubMed: 33859416
- Shi J et al. MTA2 sensitizes gastric cancer cells to PARP inhibition by induction of DNA replication stress. Transl Oncol 14:101167 (2021). PubMed: 34280886
