Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-MT2-MMP抗体[MM0028-5D11] (ab56308)

Submit a review Q&A (2)References (1)
Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)

Key features and details

  • Mouse monoclonal [MM0028-5D11] to MT2-MMP
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MT2-MMP抗体[MM0028-5D11]

  • 描述

    小鼠单克隆抗体[MM0028-5D11] to MT2-MMP

  • 宿主

    Mouse

  • 特异性

    ab56308 recognizes human MT2-MMP in western blot without cross reactivity with human MMP14/16. Reactivity with MT2-MMP in other species has not been tested.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human MT2-MMP.

  • 阳性对照

    • WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab56308于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/400 - 1/1000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/200.
说明
WB
1/400 - 1/1000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/200.

靶标

  • 功能

    Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

  • 组织特异性

    Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.

  • 序列相似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻译后修饰

    The precursor is cleaved by a furin endopeptidase.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI503551 antibody
      • Matrix metallopeptidase 15 membrane inserted antibody
      • Matrix metalloproteinase 15 antibody
      • Matrix metalloproteinase-15 antibody
      • Membrane type 2 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 2 antibody
      • Membrane-type matrix metalloproteinase 2 antibody
      • Membrane-type-2 matrix metalloproteinase antibody
      • MMP 15 antibody
      • MMP-15 antibody
      • MMP15 antibody
      • MMP15_HUMAN antibody
      • MT MMP 2 antibody
      • MT-MMP 2 antibody
      • MT2 MMP antibody
      • MT2-MMP antibody
      • MT2MMP antibody
      • MTMMP 2 antibody
      • MTMMP2 antibody
      • SMCP 2 antibody
      • SMCP-2 antibody
      • SMCP2 antibody
      see all

    图片

    • Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Anti-MT2-MMP antibody [MM0028-5D11] (ab56308) at 1/400 dilution + Human placenta tissue lysate

      Predicted band size: 76 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)

      ab56308 at 1/200 dilution, staining MT2-MMP in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    文献 (1)

    发表研究结果有使用 ab56308？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab56308 被引用在 1 文献中.

    • Yi G  et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

     Hi,   I’m wondering how long you suggest an antibody lasts for – I purchased several antibodies about eight months ago and none of them came with an expiry date, the info sheets just say “store at -20 long term”.  These antibodies are:   AB38971 Rabbit Polyclonal to MMP14 - Hinge region Lot # GR12166 AB56308 Mouse Monoclonal [MM0028-5D11] to MMP15 Lot #GR40525 AB73877 Rabbit Polyclonal to MMP16 Lot # GR30734 AB51075 Rabbit Monoclonal [EP1270Y] to MMP17 Lot # GR19885 AB39031 Rabbit Polyclonal to MMP25 - Hinge region Lot # 275516   Thank you,      

    Read More
    Answer

    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Dear Abcam, I was hoping that you could provide me with some more detail about the mouse monoclonal antibody targeting MMP-15 (AB56308). I am after some information regarding the immunogen used to create this antibody or at least the region of MMP-15 that it targets. Thank you for your help.

    Read More
    Answer

    Thank you for contacting us. I was informed that it was tested to pick up the extracellular domain of the recombinant protein. Unfortunately, we do not have the detailed epitope information available. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com