Anti-MST抗体(ab224043)
Key features and details
- Rabbit polyclonal to MST
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MST抗体
参阅全部 MST 一抗 -
描述
兔多克隆抗体to MST -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICCmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MST aa 100 to the C-terminus.
Database link: P25325 -
阳性对照
- IHC: Human liver and kidney tissue. WB: HEK-293 cell lysate. ICC: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.
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| ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa. |
|
ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis. -
序列相似性
Contains 2 rhodanese domains. -
结构域
The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4357 Human
- Omim: 602496 Human
- SwissProt: P25325 Human
- Unigene: 248267 Human
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别名
- 3 mercaptopyruvate sulfurtransferase antibody
- 3-mercaptopyruvate sulfurtransferase antibody
- Human liver rhodanese antibody
see all
图片
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Western blot - Anti-MST antibody (ab224043)All lanes : Anti-MST antibody (ab224043) at 0.04 µg/ml
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 33 kDaWestern blot analysis labeling MST with ab224043 at 0.04 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)Paraffin-embedded human liver tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)Paraffin-embedded human kidney tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry - Anti-MST antibody (ab224043)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MST (green) using ab224043 at 4 µg/ml in ICC.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab224043 被引用在 5 文献中.
- Liu H et al. Hydrogen sulfide inhibits human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG4B. J Med Virol 95:e28176 (2023). PubMed: 36163615
- Ascenção K et al. Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells. Redox Biol 56:102466 (2022). PubMed: 36113340
- Carter RN et al. The hepatic compensatory response to elevated systemic sulfide promotes diabetes. Cell Rep 37:109958 (2021). PubMed: 34758301
- Hao YM et al. Association of Hydrogen Sulfide with Femoral Bone Mineral Density in Osteoporosis Patients: A Preliminary Study. Med Sci Monit 27:e929389 (2021). PubMed: 33714972
- Hu HJ et al. Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress. Front Pharmacol 12:690371 (2021). PubMed: 34950023