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AB3322

Anti-MSS1抗体

Anti-MSS1 antibody

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(6 Publications)

Rabbit Polyclonal MSS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PSMC2.

查看别名

MSS1, PSMC2, 26S proteasome regulatory subunit 7, 26S proteasome AAA-ATPase subunit RPT1, Proteasome 26S subunit ATPase 2

2 Images
Western blot - Anti-MSS1 antibody (AB3322)
  • WB

Unknown

Western blot - Anti-MSS1 antibody (AB3322)

Western blot of MSS1 on HeLa cell lysate using ab3322.

All lanes:

Western blot - Anti-MSS1 antibody (ab3322)

Predicted band size: 49 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-MSS1 antibody (AB3322)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSS1 antibody (AB3322)

Immunofluorescence analysis of MSS1 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSS1 Rabbit Polyclonal Antibody (ab3322) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin (1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human PSMC2.

P35998

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯度
IgG fraction
存储溶液
Constituents: 99% PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MSS1 also known as Mitochondrial Stress Sensitive 1 is a protein that plays a role in cellular processes. It has an approximate mass of 27 kDa. MSS1 expresses in various tissues with higher levels observed in mitochondria-rich tissues indicating a role in energy metabolism. Primarily the protein operates within the mitochondria where it takes part in maintaining cellular homeostasis.
Biological function summary

MSS1 contributes to the regulation of mitochondrial function and integrity. It is involved in the formation and maintenance of mitochondrial membranes and assists in mitochondrial protein translocation. As part of the TIM22 complex MSS1 is responsible for the insertion and assembly of membrane proteins into the inner mitochondrial membrane playing a role in efficient energy production and overall cellular respiration.

Pathways

MSS1 contributes significantly to the oxidative phosphorylation and mitochondrial protein import pathways. Within these pathways it interacts with the TIM22 complex facilitating the integration of translocase-associated proteins into the inner membrane. This interaction is important for maintaining mitochondrial structure and function supporting energy metabolism in cells. Additionally MSS1's function closely relates to proteins like TIM23 and TIM44 which also help in protein translocation and integration within the mitochondria.

MSS1 is linked to conditions such as mitochondrial myopathy and mitochondrial encephalomyopathy. These disorders often involve defects in mitochondrial function and energy metabolism. Anomalies in MSS1 expression or function could impact the integration of proteins into the mitochondrial membrane leading to impaired mitochondrial function. MSS1's role in these disorders often involves interaction with other mitochondrial import machinery components like TIM44 which may also influence pathological outcomes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
See full target information PSMC2
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 2: PubMed31431473

2019

A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Serge Auvin,Harun Öztürk,Yusuf T Abaci,Gisele Mautino,Florence Meyer-Losic,Florence Jollivet,Tarig Bashir,Hugues de Thé,Umut Sahin

PloS one 9:e105947 PubMed25153908

2014

Fusion-related host proteins are actively regulated by NA during influenza infection as revealed by quantitative proteomics analysis.

Applications

WB

Species

Dog

Zhiwei Sui,Bo Wen,Zhimin Gao,Quanjiao Chen

PloS one 9:e91200 PubMed24625964

2014

Nonproteolytic roles of 19S ATPases in transcription of CIITApIV genes.

Applications

Unspecified application

Species

Human

Nagini Maganti,Tomika D Moody,Agnieszka D Truax,Meghna Thakkar,Alexander M Spring,Markus W Germann,Susanna F Greer

The Journal of biological chemistry 287:4790-9 PubMed22194612

2011

Competition between sumoylation and ubiquitination of serine hydroxymethyltransferase 1 determines its nuclear localization and its accumulation in the nucleus.

Applications

ICC/IF

Species

Human

Donald D Anderson,Jae Y Eom,Patrick J Stover

Molecular endocrinology (Baltimore, Md.) 25:394-408 PubMed21273440

2011

Ligand-dependent degradation of SRC-1 is pivotal for progesterone receptor transcriptional activity.

Applications

ICC/IF

Species

Human

Larbi Amazit,Audrey Roseau,Junaid A Khan,Anne Chauchereau,Rakesh K Tyagi,Hugues Loosfelt,Philippe Leclerc,Marc Lombès,Anne Guiochon-Mantel

The Journal of biological chemistry 284:1917-29 PubMed18986984

2008

Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones.

Applications

ICC/IF, WB, ICC/IF, WB

Species

Human, Human, Rat, Rat

Erwann Rousseau,Rieko Kojima,Guylaine Hoffner,Philippe Djian,Anne Bertolotti
View all publications
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