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AB313332

重组Anti-MSH4抗体[EPR27244-57] - BSA and Azide free

Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free

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Rabbit Recombinant Monoclonal MSH4 antibody. Carrier free. Suitable for WB, IP and reacts with Mouse samples.

查看别名

MutS protein homolog 4, mMsh4, Msh4

2 Images
Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free (AB313332)
  • IP

Supplier Data

Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free (AB313332)

This data was developed using ab313331, the same antibody clone in a different buffer formulation. MSH4 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab313331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse testis tissue lysate Lane 2 : Mouse testis tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313331 in mouse testis tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 58 seconds

All lanes:

Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] (<a href='/products/primary-antibodies/msh4-antibody-epr27244-57-ab313331'>ab313331</a>) at 1/30 dilution

All lanes:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 58s

Western blot - Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free (AB313332)
  • WB

Supplier Data

Western blot - Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free (AB313332)

This data was developed using ab313331, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : spleen (PMID : 9299235, 10809667, 18947432 ), kidney (PMID : 10809667, 18947432). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-MSH4 antibody [EPR27244-57] (<a href='/products/primary-antibodies/msh4-antibody-epr27244-57-ab313331'>ab313331</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR27244-57

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: 100% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MSH4 known as MutS Homolog 4 is a protein involved in meiotic recombination and DNA repair. MSH4 is a member of the DNA mismatch repair (MMR) system which is known for its role in maintaining genome stability. The protein has an estimated mass of around 103 kDa. MSH4 is expressed in the testis and ovary where it localizes to the synaptonemal complex during the early stages of meiosis. It assists in the proper alignment and pairing of homologous chromosomes essential for accurate genetic recombination.
Biological function summary

MSH4 contributes to genome diversity and stability by facilitating the formation and resolution of crossover events during meiosis I. MSH4 forms a heterodimer with MSH5 another MutS homolog which is necessary for recognizing and binding to DNA double-strand breaks. This complex acts as a scaffold for the assembly of other repair proteins required for recombination. The interaction of MSH4-MSH5 complex with important proteins like MLH1 and MLH3 fosters the maturation of crossovers further ensuring genetic diversity and error-free distribution of genetic material.

Pathways

MSH4 plays an important role in meiotic homologous recombination and mismatch repair pathways. Within the homologous recombination pathway MSH4 operates with MSH5 to promote synapsis and crossover formation between homologous chromosomes. This involvement is critical for fertility and genome stability. The protein also interacts with the MLH1 and MLH3 in the repair pathway emphasizing its role in the maintenance and integrity of genetic material during cell division.

Defects in the MSH4 function can lead to anomalies in meiotic recombination increasing the risk for infertility and certain cancers. It has been associated with conditions such as azoospermia where dysfunctional MSH4 may result in improper chromosomal segregation. The interaction of MSH4-MSH5 with other proteins like MLH1 also relates to Lynch syndrome characterized by increased cancer propensity due to mismatch repair deficiencies. Therefore understanding MSH4 and its connections with other proteins is essential for researching therapeutic interventions in these conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.
See full target information Msh4
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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关联产品

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Alternative Version
Primary Antibodies

AB313331

Anti-MSH4 antibody [EPR27244-57]

primary-antibodies

msh4-antibody-epr27244-57-ab313331

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