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AB275928

重组Anti-MSH3抗体[RM405]

Anti-MSH3 antibody [RM405]

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(3 Publications)

Anti-MSH3 antibody [RM405] (ab275928) is a rabbit monoclonal antibody detecting MSH3 in Western Blot, IHC-P. Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Recombinant format for unrivalled batch-batch consistency

查看别名

DUC1, DUG, MSH3, DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, Mismatch repair protein 1, DUP, MRP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 antibody [RM405] (AB275928)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 antibody [RM405] (AB275928)

Formalin-fixed, paraffin-embedded human colon cancer tissue stained for MSH3 using ab275928 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-MSH3 antibody [RM405] (AB275928)
  • WB

Supplier Data

Western blot - Anti-MSH3 antibody [RM405] (AB275928)

All lanes:

Western blot - Anti-MSH3 antibody [RM405] (ab275928) at 1/800 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Predicted band size: 127 kDa

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Western blot - Anti-MSH3 antibody [RM405] (AB275928)
  • WB

Lab

Western blot - Anti-MSH3 antibody [RM405] (AB275928)

False colour image of Western blot : Anti-MSH3 antibody [RM405] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275928 was shown to bind specifically to MSH3. A band was observed at 140 kDa in wild-type A549 cell lysates with no signal observed at this size in MSH3 knockout cell line ab288875. To generate this image, wild-type and MSH3 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MSH3 antibody [RM405] (ab275928) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

MSH3 knockout A549 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 127 kDa

Observed band size: 140 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

RM405

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB92471

Anti-MSH6 antibody [EPR3945]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (AB92471)

  • 5
  • 5
Primary Antibodies

AB92312

Anti-MLH1 antibody [EPR3894]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (AB92312)

  • 5
  • 4
Primary Antibodies

AB70270

Anti-MSH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)

  • 5
  • 4
Primary Antibodies

AB110638

Anti-PMS2 antibody [EPR3947]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] (AB110638)

  • 4
  • 3
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-MSH3 antibody [RM405] (ab275928) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of MSH3?
Anti-MSH3 [RM405] (ab275928) specifically detects a band for MSH3 (UniProt: P20585) at a molecular weight of 127kDa.

Specificity confirmed
The specificity of Anti-MSH3 antibody [RM405] (ab275928) has been confirmed by Western blot testing in MSH3 Knockout A549 cell line, ab288875.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The mutS homolog 3 (MSH3) protein plays an important role in DNA mismatch repair. It is part of the family of MutS proteins and is sometimes referred to by its gene name MSH3. MSH3 forms a heterodimer with MSH2 to create MutSΒ. This complex targets insertion and deletion loops. The mass of MSH3 is approximately 127 kDa. Expression of MSH3 occurs in various tissues with high levels in the colon small intestine and testis.
Biological function summary

MSH3 contributes to genome stability by correcting DNA replication errors. As part of the MutSΒ complex it identifies and initiates repair of insertion and deletion loops which helps maintain genomic integrity. In eukaryotes MSH3 along with its partner MSH2 cooperates with other proteins such as MLH1 and PMS2 to ensure proper mismatch repair. These interactions help to prevent mutations from becoming permanent.

Pathways

MSH3 participates in the DNA mismatch repair pathway important for maintaining genomic fidelity. It works closely with MSH2 to form MutSΒ enhancing the repair of small loops. Another important association includes the MLH1-PMS2 complex within the mismatch repair pathways. This collaboration ensures errors in DNA during replication do not lead to genomic instability.

Mutations or malfunctioning of MSH3 have been associated with colorectal cancer and Lynch syndrome. Alterations in MSH3 can impair DNA repair processes contributing to cancer development. The MSH2 protein which pairs with MSH3 also plays a critical role in these disorders. Understanding MSH3 function helps illuminate its involvement in genetic stability and its impact on health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
See full target information MSH3
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:113866 PubMed38416638

2024

Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation.

Applications

Unspecified application

Species

Unspecified reactive species

Harry Warner,Giulia Franciosa,Guus van der Borg,Britt Coenen,Felix Faas,Claire Koenig,Rinse de Boer,René Classens,Sjors Maassen,Maksim V Baranov,Shweta Mahajan,Deepti Dabral,Frans Bianchi,Niek van Hilten,Herre Jelger Risselada,Wouter H Roos,Jesper Velgaard Olsen,Laia Querol Cano,Geert van den Bogaart

Nature communications 14:505 PubMed36720864

2023

Proteogenomics of diffuse gliomas reveal molecular subtypes associated with specific therapeutic targets and immune-evasion mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Wang,Rongkui Luo,Xuan Zhang,Hang Xiang,Bing Yang,Jinwen Feng,Mengjie Deng,Peng Ran,Akesu Sujie,Fan Zhang,Jiajun Zhu,Subei Tan,Tao Xie,Pin Chen,Zixiang Yu,Yan Li,Dongxian Jiang,Xiaobiao Zhang,Jian-Yuan Zhao,Yingyong Hou,Chen Ding

EBioMedicine 69:103436 PubMed34157484

2021

Identification of ARGLU1 as a potential therapeutic target for gastric cancer based on genome-wide functional screening data.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Li,Jianfang Li,Junxian Yu,Tao Pan,Beiqin Yu,Qingqing Sang,Wentao Dai,Junyi Hou,Chao Yan,Mingde Zang,Zhenggang Zhu,Liping Su,Yuan-Yuan Li,Bingya Liu
View all publications
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