AB271068

重组Anti-MS4A2抗体[EPR24324-7]

Anti-MS4A2 antibody [EPR24324-7]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MS4A2 antibody. Suitable for ICC/IF, IP, Dot, IHC-P and reacts with Human, Synthetic peptide - Human samples.

查看别名

APY, FCER1B, IGER, MS4A2, High affinity immunoglobulin epsilon receptor subunit beta, FcERI, Fc epsilon receptor I beta-chain, IgE Fc receptor subunit beta, Membrane-spanning 4-domains subfamily A member 2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human prostatic hyperplasia. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human breast carcinoma. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling MS4A2 with ab271068 at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) antibody at 1/500 dilution (Red).

Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged MS4A2 expression vector (PMID : 18571665).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 488) was used to counterstain tubulin at 1/100 dilution (Green). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human colon. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

MS4A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with MS4A2 expression vector, whole cell lysate 10 μg with ab271068 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271068 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T transfected with MS4A2 expression vector, whole cell lysate 10 μg

Lane 2 : ab271068 IP in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271068 in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 7.75 seconds

All lanes:

Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] (ab271068)

Predicted band size: 27 kDa

false

Supplier Data

Dot Blot - Anti-MS4A2 antibody [EPR24324-7] (AB271068)

Dot blot analysis of MS4A2 using ab271068 at 1 : 1000 dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Human MS4A2 peptide (aa 226-243)
Lane 2 : Human MS4A2 peptide (aa 206-223)
Lane 3 : Human MS4A2 peptide (aa 151-171)

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST

不同偶联物与剂型 (1)

Carrier free

Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24324-7

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, Dot, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Synthetic peptide - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MS4A2 also known as FcεRIβ or High-affinity IgE receptor beta subunit weighs approximately 30 kDa. This protein is expressed mostly in mast cells and basophils. MS4A2 functions as part of the high-affinity IgE receptor complex which is critical in mediating immune responses that are rapid and strong. In terms of structure the MS4A family consists of proteins with four transmembrane domains including MS4A2 playing a role in signal transduction processes.

Biological function summary

MS4A2 contributes significantly to the immune system by participating in allergic response signaling. It assembles with other subunits — alpha and gamma — to form the tetrameric IgE receptor complex. This complex on mast cells and basophils detects IgE antibodies bound to allergens triggering the release of inflammatory mediators like histamine. These mediators lead to symptoms associated with allergic reactions such as asthma and anaphylaxis. MS4A2's interaction with allergens through the receptor complex makes it an essential player in immune surveillance and response.

Pathways

MS4A2 directly influences the Fc epsilon RI signaling pathway an essential component in immune responses especially related to allergy and hypersensitivity reactions. The pathway not only involves MS4A2 but also proteins like Lyn and Syk kinase which further relay the signals initiated by antigen-IgE binding. This interaction can lead to robust downstream immune responses including inflammatory processes. The MS4A2 protein's role in this pathway connects it with other signaling cascades enhancing cell activation and response.

Research identifies MS4A2's relevance in allergic asthma and atopic dermatitis. These conditions often involve an overactive immune response to environmental allergens where MS4A2 plays a central role. In these diseases the interaction of MS4A2 with other molecules such as IL-4 and IL-13 reveals its influence on disease pathology. This understanding helps in considering potential therapeutic targets as MS4A2's contribution to disease progression offers a path to developing treatments focused on modulating allergic response pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

High affinity receptor that binds to the Fc region of immunoglobulins epsilon. Aggregation of FCER1 by multivalent antigens is required for the full mast cell response, including the release of preformed mediators (such as histamine) by degranulation and de novo production of lipid mediators and cytokines. Also mediates the secretion of important lymphokines. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators responsible for the manifestations of allergy.

See full target information MS4A2

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MS4A2抗体[EPR24324-7]