Anti-MRPS7抗体(ab224442)
Key features and details
- Rabbit polyclonal to MRPS7
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MRPS7抗体
参阅全部 MRPS7 一抗 -
描述
兔多克隆抗体to MRPS7 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human MRPS7 aa 50-200.
Database link: Q9Y2R9 -
阳性对照
- IHC-P: Human liver tissue. WB: NIH/3T3 and NBT-II cell lysates. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224442于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 28 kDa.
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 28 kDa. |
靶标
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序列相似性
Belongs to the ribosomal protein S7P family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 509115 Cow
- Entrez Gene: 51081 Human
- Entrez Gene: 50529 Mouse
- Entrez Gene: 113958 Rat
- Omim: 611974 Human
- SwissProt: Q3T040 Cow
- SwissProt: Q9Y2R9 Human
- SwissProt: Q80X85 Mouse
see all -
别名
- 28S ribosomal protein S7 antibody
- 28S ribosomal protein S7, mitochondrial precursor antibody
- 30S ribosomal protein S7 homolog antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS7 antibody (ab224442)
Paraffin-embedded human liver tissue stained for MRPS7 using ab224442 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-MRPS7 antibody (ab224442)All lanes : Anti-MRPS7 antibody (ab224442) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 28 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MRPS7 antibody (ab224442)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MRPS7 (green) using ab224442 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224442 尚未被引用在任何文献中。