Anti-MRPS22抗体(ab224341)
Key features and details
- Rabbit polyclonal to MRPS22
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MRPS22抗体 -
描述
兔多克隆抗体to MRPS22 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human MRPS22 aa 50-200.
Database link: P82650 -
阳性对照
- WB: RT4, U-251 MG sp, NIH/3T3 and NBT-II cell lysates. IHC-P: Human lymph node tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224341于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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疾病相关
Combined oxidative phosphorylation deficiency 5 -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 56945 Human
- Entrez Gene: 64655 Mouse
- Entrez Gene: 683519 Rat
- Omim: 605810 Human
- SwissProt: P82650 Human
- SwissProt: Q9CXW2 Mouse
- Unigene: 745001 Human
- Unigene: 17949 Mouse
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别名
- 28S ribosomal protein S22 antibody
- C3orf5 antibody
- COXPD5 antibody
see all
图片
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Western blot - Anti-MRPS22 antibody (ab224341)All lanes : Anti-MRPS22 antibody (ab224341) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Predicted band size: 41 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS22 antibody (ab224341)Paraffin-embedded human lymph node tissue stained for MRPS22 using ab224341 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-MRPS22 antibody (ab224341)All lanes : Anti-MRPS22 antibody (ab224341) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 41 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MRPS22 antibody (ab224341)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MRPS22 (green) using ab224341 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224341 尚未被引用在任何文献中。