AB180165

重组Anti-MRPL4抗体[EPR13151]

Anti-MRPL4 antibody [EPR13151]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal MRPL4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

CDABP0091, CGI-28, MRPL4, Large ribosomal subunit protein uL4m, L4mt, MRP-L4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] (AB180165)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] (AB180165)

Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labeling MRPL4 using ab180165 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] (AB180165)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling MRPL4 using ab180165 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MRPL4 antibody [EPR13151] (AB180165)

All lanes:

Western blot - Anti-MRPL4 antibody [EPR13151] (ab180165) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HL60 cell lysate at 10 µg

Lane 4:

HT29 cell lysate at 10 µg

Predicted band size: 35 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13151

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MRPL4 also known as 39S ribosomal protein L4 mitochondrial is a mitochondrial ribosomal protein. This protein is a component of the large 39S subunit of the mitochondrial ribosome and has a molecular mass of approximately 39 kDa. MRPL4 is primarily expressed in tissues with high energy demands including the heart and skeletal muscle. It plays a role in mitochondrial protein synthesis facilitating the translation of proteins encoded by mitochondrial DNA.

Biological function summary

MRPL4 assists in the assembly and stability of the mitochondrial ribosome forming part of the mitoribosome complex. This complex is essential for the synthesis of mitochondrial proteins which are critical for proper mitochondrial function. MRPL4 ensures accurate translation by binding to ribosomal RNA stabilizing the structure needed for efficient ribosomal activity. Its function is important for maintaining cellular energy production cellular growth and metabolism.

Pathways

MRPL4 participates significantly in the mitochondrial translation pathway. This pathway is integral to the oxidative phosphorylation process which is essential for ATP production in cells. MRPL4 interacts with other ribosomal proteins such as MRPL3 and MRPL12 facilitating the coordination of protein synthesis within the mitochondrion. This process influences cellular respiration and bioenergetics which are critical for adapting to energy requirements.

MRPL4 is associated with conditions like mitochondrial disorders and cardiac diseases. Alterations in MRPL4 expression or function can disrupt mitochondrial protein synthesis leading to impaired oxidative phosphorylation and energy production. Such mitochondrial dysfunction is linked to disorders like mitochondrial encephalomyopathy and cardiomyopathy. MRPL4's interactions with other ribosomal proteins ensure that changes in its structure or expression could influence disease progression making it a target for further research in mitochondrial medicine.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information MRPL4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical jo 22:2357-62 PubMed27385084

2016

Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Su Tang,Xiaodong Tang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MRPL4抗体[EPR13151]

Anti-MRPL4 antibody [EPR13151]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] (AB180165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] (AB180165)

Western blot - Anti-MRPL4 antibody [EPR13151] (AB180165)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原