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重组RabMAb

重组Anti-MRPL28抗体[EPR7578(B)] (ab126719)

Reviews (1)Q&A (2)References (1)
Western blot - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
  • OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
  • Anti-MRPL28 antibody [EPR7578(B)] (ab126719)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7578(B)] to MRPL28
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MRPL28抗体[EPR7578(B)]
    参阅全部 MRPL28 一抗

  • 描述

    兔单克隆抗体[EPR7578(B)] to MRPL28

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Fetal kidney tissue lysates JAR cell lysates HepG2 whole cell lysate (ab7900) A549 cell lysates 293T cell lysates A549 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126719于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
ICC/IF
1/250 - 1/500.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
ICC/IF
1/250 - 1/500.
应用说明
Is unsuitable for IHC-P.

靶标

  • 组织特异性

    Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.

  • 序列相似性

    Belongs to the ribosomal protein L28P family.

  • 细胞定位

    Mitochondrion.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 39S ribosomal protein L28 antibody
      • 39S ribosomal protein L28 mitochondrial antibody
      • 39S ribosomal protein L28 mitochondrial precursor antibody
      • HGNC6756 antibody
      • L28mt antibody
      • MAAT 1 antibody
      • MAAT1 antibody
      • Melanoma antigen p15 antibody
      • Melanoma associated antigen recognised by cytotoxic T lymphocytes antibody
      • Melanoma associated antigen recognized by T lymphocytes antibody
      • Melanoma-associated antigen recognized by T-lymphocytes antibody
      • MGC8499 antibody
      • mitochondrial antibody
      • Mitochondrial ribosomal protein L28 antibody
      • MRP L28 antibody
      • MRP-L28 antibody
      • MRPL 28 antibody
      • mrpl28 antibody
      • P15 antibody
      • RM28_HUMAN antibody
      see all

    图片

    • Western blot - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      Western blot - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      All lanes : Anti-MRPL28 antibody [EPR7578(B)] (ab126719) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : 293T (Human embryonic kidney epithelial cell) cell lysate

      Predicted band size: 30 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.
    • OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-MRPL28 antibody [EPR7578(B)] (ab126719)
      Anti-MRPL28 antibody [EPR7578(B)] (ab126719)

    文献 (1)

    发表研究结果有使用 ab126719？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab126719 被引用在 1 文献中.

    • Schneider K  et al. Protein Stability Buffers the Cost of Translation Attenuation following eIF2a Phosphorylation. Cell Rep 32:108154 (2020). PubMed: 32937139

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-MRPL28 antibody [EPR7578(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    6.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 26 2015

    Question

    Dear ,
    Thanks for the detailed information.
    I believe that the product ab126719 fits perfect to our experiments.
    Please, let me know if there is any company that represents Abcam in Norway.
    Regards,

    Read More
    Answer

    Thank you for your reply. I am glad I could help you choose the best antibody for your experiment.

    We do not have any distributor in Norway, you can order directly from us. Please let me know if you need a proforma and I will be happy to send it to you.

    If not, you can order directly through mailto:orders@abcam.com indicating your billing and shipping address as well as a valid VAT number.

    If you need further assistance, please do not hesitate to contact us back.

    Read More

    Question

    Dear,
    I would like to know if one of the following antibodies can be used for ether human, rat and mice wester blot? Or, do you have any other antibody against human, rat and mice MRPL28?
    Anti-MRPL28 antibody (ab15871)
    Anti-MRPL28 antibody (ab29745)
    Regards,

    Read More
    Answer

    Thank you for contacting us.

    There are four antibodies in our catalogue suitable for WB in either one of the mentioned species.

    Product ab126719 is a rabbit monoclonal that has been validated by WB in Human, Mouse and Rat, you can have a look at the blot image on its datasheet:

    https://www.abcam.com/MRPL28-antibody-EPR7578B-ab126719.html

    Alternatively, https://www.abcam.com/MRPL28-antibody-ab89030.html and https://www.abcam.com/MRPL28-antibody-ab15871.html are also suitable for WB in Human:

    https://www.abcam.com/MRPL28-antibody-ab89030.html

    https://www.abcam.com/MRPL28-antibody-ab15871.html

    And ab98922 has been tested in Human, but due to sequence homology it is also expected to react with Mouse and Rat:

    https://www.abcam.com/MRPL28-antibody-ab98922.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com