Anti-MRPL18抗体(ab67844)
Key features and details
- Mouse polyclonal to MRPL18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MRPL18抗体 -
描述
小鼠多克隆抗体to MRPL18 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human MRPL18. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human liver tissue lysate, transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa). |
靶标
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序列相似性
Belongs to the ribosomal protein L18P family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 29074 Human
- Omim: 611831 Human
- SwissProt: Q9H0U6 Human
- Unigene: 416998 Human
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别名
- 39S ribosomal protein L18 antibody
- 39S ribosomal protein L18 mitochondrial antibody
- 39S ribosomal protein L18, mitochondrial precursor antibody
see all
图片
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Western blot - Anti-MRPL18 antibody (ab67844)Anti-MRPL18 antibody (ab67844) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Western blot - Anti-MRPL18 antibody (ab67844)All lanes : Anti-MRPL18 antibody (ab67844) at 1/500 dilution
Lane 1 : MRPL18 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (3)
ab67844 被引用在 3 文献中.
- Naesens L et al. GTF3A mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand RNA5SP141. Sci Immunol 7:eabq4531 (2022). PubMed: 36399538
- Chiang JJ et al. Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity. Nat Immunol 19:53-62 (2018). PubMed: 29180807
- Smirnov A et al. Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18. Genes Dev 25:1289-305 (2011). WB ; Human . PubMed: 21685364
