AB181834

重组Anti-MRPL16抗体[EPR14351]

Anti-MRPL16 antibody [EPR14351]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal MRPL16 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

PNAS-111, MRPL16, Large ribosomal subunit protein uL16m, L16mt, MRP-L16

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL16 antibody [EPR14351] (AB181834)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL16 antibody [EPR14351] (AB181834)

Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling MRPL16 using ab181834 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MRPL16 antibody [EPR14351] (AB181834)

Blocking/ Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MRPL16 antibody [EPR14351] (ab181834) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-MRPL16 antibody [EPR14351] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14351

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MRPL16 also known as mitochondrial ribosomal protein L16 is a part of the mitochondrial ribosome which helps synthesize proteins within the mitochondria. MRPL16 has a molecular weight of around 36 kDa. Researchers find this protein in most tissues but it is especially abundant in energy-demanding tissues like the heart and skeletal muscles. As part of the large subunit of the mitochondrial ribosome MRPL16 interacts with other ribosomal proteins to help maintain functions in protein biosynthesis.

Biological function summary

The mitochondrial ribosome plays an important role in facilitating mitochondrial protein synthesis by translating mitochondrial mRNAs into proteins. MRPL16 acts as a component of this ribosome which consists of numerous proteins and ribosomal RNA (rRNA). This mitochondrial ribosomal protein aids in stabilizing the structure and function of the ribosome ensuring accurate translation of genetic information within mitochondria. Through this function MRPL16 supports cellular respiration and energy production by maintaining the synthesis of essential proteins required for mitochondrial function.

Pathways

The mitochondrial protein synthesis pathway is essential for producing key proteins involved in oxidative phosphorylation the main ATP-generating mechanism in cells. MRPL16 through its association with the ribosomal complex plays a role in coordinating the production of proteins in the respiratory chain complexes. Proteins like cytochrome c oxidase and ATP synthase depend on the mitochondrial ribosome's proper function. MRPL16 therefore indirectly supports these pathways as anomalies in mitochondrial protein synthesis can disrupt ATP production.

MRPL16 has links to conditions involving mitochondrial dysfunction such as mitochondrial myopathy and Leigh syndrome. Defects in mitochondrial protein synthesis and energy metabolism often lead to these disorders. The protein's association with other ribosomal proteins like MRPL21 and MRPL11 becomes significant as disruptions in these proteins can lead to misfolding or incomplete synthesis of critical mitochondrial proteins. Understanding MRPL16's role helps us study how mitochondrial dysfunction contributes to these diseases and devise targeted therapies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information MRPL16

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Acta ophthalmologica 102:773-778 PubMed38553957

2024

Effect of chlorhexidine, povidone-iodine and betadine antiseptic eye drops on cultured human conjunctival goblet cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Rubin Hadad,Anne Hedengran,Alex Barnils,Goran Petrovski,Barbara Cvenkel,Tor P Utheim,Darlene A Dartt,Steffen Heegaard,Miriam Kolko

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MRPL16抗体[EPR14351]