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Anti-MRP3抗体[M3II-9] (ab3375)

Reviews (3)Q&A (2)References (14)
Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)

    Key features and details

    • Mouse monoclonal [M3II-9] to MRP3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-MRP3抗体[M3II-9]
      参阅全部 MRP3 一抗

    • 描述

      小鼠单克隆抗体[M3II-9] to MRP3

    • 宿主

      Mouse

    • 特异性

      This antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Recombinant fragment within Human MRP3 aa 800-950. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: O15438-1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 表位

      This antibody detects with an internal epitope of the membrane transport protein of MRP3

    • 阳性对照

      • Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives

    • 常规说明

      The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3375于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (3)
    1/20 - 1/50. Detects a band of approximately 190 kDa.
    说明
    WB
    1/20 - 1/50. Detects a band of approximately 190 kDa.

    靶标

    • 功能

      May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.

    • 组织特异性

      Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

    • 序列相似性

      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ABC 31 antibody
        • ABC31 antibody
        • ABCC 3 antibody
        • ABCC3 antibody
        • ATP binding cassette sub family C (CFTR/MRP) member 3 antibody
        • ATP binding cassette sub family C member 3 antibody
        • ATP-binding cassette sub-family C member 3 antibody
        • Canalicular multispecific organic anion transporter 2 antibody
        • Canicular multispecific organic anion transporter antibody
        • CMOAT 2 antibody
        • CMOAT2 antibody
        • EST90757 antibody
        • MLP 2 antibody
        • MLP2 antibody
        • MOAT D antibody
        • MOAT-D antibody
        • MOATD antibody
        • MRP 3 antibody
        • MRP3_HUMAN antibody
        • Multi specific organic anion transporter D antibody
        • Multi-specific organic anion transporter D antibody
        • Multidrug resistance associated protein 3 antibody
        • Multidrug resistance associated protein antibody
        • Multidrug resistance-associated protein 3 antibody
        • Multispecific organic anion tranporter D antibody
        see all

      图片

      • Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)
        Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)

         HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

        Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

      文献 (14)

      发表研究结果有使用 ab3375？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3375 被引用在 14 文献中.

      • Lahmar A  et al. Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum, Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein. Nutrients 13:N/A (2021). PubMed: 34069490
      • Xu F  et al. MicroRNA-448 overexpression inhibits fibroblast proliferation and collagen synthesis and promotes cell apoptosis via targeting ABCC3 through the JNK signaling pathway. J Cell Physiol 235:1374-1385 (2020). PubMed: 31506947
      • Wei W  et al. The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1. Oncol Res 28:423-438 (2020). PubMed: 32331534
      • Shi B  et al. Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncol Lett 20:148 (2020). PubMed: 32934716
      • Han SH  et al. Prognostic implication of ABC transporters and cancer stem cell markers in patients with stage III colon cancer receiving adjuvant FOLFOX-4 chemotherapy. Oncol Lett 17:5572-5580 (2019). PubMed: 31186779
      View all Publications for this product

      客户评价及客户问答

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      提交评价 提交问题

      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Liver)
      Loading amount
      80 µg
      Specification
      Liver
      Gel Running Conditions
      Non-reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 06 2010

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Liver homogenate)
      Loading amount
      25 µg
      Specification
      Liver homogenate
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 17 2009

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (lung carcinoma)
      Loading amount
      25 µg
      Specification
      lung carcinoma
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 19 2009

      Question

      To technical support,



      I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive controls.







      https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html



      https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html





      https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html



      https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html





      https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html



      https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html





      https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html



      https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html





      https://www.abcam.com/BCRPABCG2-antibody-ab63907.html



      https://www.abcam.com/BCRPABCG2-antibody-ab63907.html





      https://www.abcam.com/MRP4-antibody-ab77184.html



      https://www.abcam.com/MRP4-antibody-ab77184.html





      https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html



      https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html





      https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html



      https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html





      https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html



      https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html





      https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html



      https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html





      https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html



      https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html







      With kind regards,

      Read More
      Answer

      Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
      I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
      https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html.
      MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
      https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html.
      With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
      https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html.
      I hope this information is helpful and wish you good luck with your research.

      Read More

      Question

      I am interested in your antibodies for BCRP (ab3380: Clone BXP-21), MDR1 (ab10333: Clone 4E3), MRP2 (ab3373: Clone M2 III-6) and MRP3 (ab3375: Clone M3II-9). As I will be using these antibodies for FACS, I would like to know whether you have any of these in a conjugated version (FITC or PE) as well? If not, do you have any matching secondairy antibodies which are conjugated with FITC or PE? Are these antibodies available at the moment? If I buy one vial of each of these antibodies in one go, will there be any special price offer? I would prefer to detect only the proteins which are present in the plasma membrane. As the MRP antibodies are reacting with an internal epitope, it will be necessary to permeabilize the plasma membrane. Do you know of any more or less selective technique to permeabilize the plasma membrane resulting in a selective staining of MRP2 and MRP3 in the plasma membrane only? I am lokoing forward to your reply. Thanks for your help!

      Read More
      Answer

      Thank you for your enquiry and for your patience. Unfortunately, none of these antibodies are available in a conjugated form but we do have a wide range of secondary antibodies to choose from (including ones conjugated to FITC and PE). At the bottom of the online datasheets for each of these primary antibodies, there is a list of compatible secondary antibodies that can be used. We unfortunately can't offer any discounts. All the information that we have regarding these antibodies is located on the online datasheets, and ab10333 and ab3373 have been tested for application in FACS but ab3380 and ab3375 have not to our knowledge. If you have any more questions, please contact us again.

      Read More

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