Anti-MRP2抗体[M2III-5] (ab15603)

Thank you for contacting us.

The note regarding mouse specificity is based on data from the following reference, which includes a description of the clone's development and characterization:

Cancer Res. 2000 Sep 15;60(18):5269-77.
PMID: 11016657
http://cancerres.aacrjournals.org/content/60/18/5269.full.pdf+html

Table 1 has a summary of species reactivity for this clone and several others.

Further characterization may have shown reactivity with other murine samples, in other applications, and this may account for the information on the datasheets of other vendors, but we do not have this data.

It is possible that there is more than one clone raised against MRP2 designated "M2III-5" but this seems less likely than the possibility of differences in testing conditions and samples across different laboratories.

I hope this is helpful. Please do not hesitate to contact us if you need any more information.

Thank you very much for your inquiry and your patience while waiting for our answer.

I have inquired with the laboratories of the polyclonal antibodies, whether the immunogen sequence is conserved with the pig sequence. This would then provide a good basis for testing the antibody.

The ab110740 has unfortunately only 79%homology with the pigsequence and we can not recommend it therefore.

Also for the ab58709, the alignment is only 62%, and we can not recommend it neither. I am very sorry.

For the monoclonal antibodies, it is more difficult to predict, as the epitopes have not been mapped. As however the overall sequence alignment is relatively good, they might have also good changes to crossreact with the pig protein. Please see here the overall alignment: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120627-135954-0702-94637034-oy

The ab3373 and the ab15603 have been raised against the human protein sequence 1339-1541. There are many conserved residues.
The ab24106 and the ab130385 have been raised against the human sequence 860-950, which seems less conserved than the C terminal. The ab3372 has been raised against human sequence 215 to 310, again, a less conserved region than the C terminus.

So in summary, I would think either the ab3373 or ab15603 most likely to crossreact with the pig protein from the choice of antibodies we have. This has however not been tested, and it can well be that they will not react with the pig protein.
https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html (or use the following: https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html).
https://www.abcam.com/MRP2-antibody-M2III-5-ab15603.html (or use the following: https://www.abcam.com/MRP2-antibody-M2III-5-ab15603.html).

If you would therefore like to test this, I can offer a discount off a future purchase if you buy ab3373 or ab15603 now, test it inpig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab3373 or ab15603 in pig. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchase ab3373 or ab15603 either by phone, fax, or online (www.abcam.com).

3. Test it in pig.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab3373 or ab15603 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for your feedback regarding this product. As a result of the information that you have sent to me and references sourced via “Pub Med” I have currently removed cross reactivity with from our product data base. I would like to thank you for the information that you have forwarded and would be more than willing to offer either a credit note to your account/ refund. Could you confirm for me the order details cited below and I will arrange a credit note to be issued? Your PO or our Abcam order number The name of your purchasing agent and the shipping address. I look forward to hearing from you soon.