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Signal Transduction Metabolism Plasma Membrane Channels
使用敲除细胞株进行验证

Anti-MRP2抗体[M2 III-6] (ab3373)

  • Datasheet
  • SDS
Reviews (7)Q&A (11)References (72)

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Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)
  • Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)

Key features and details

  • Mouse monoclonal [M2 III-6] to MRP2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-MRP2抗体[M2 III-6]
    参阅全部 MRP2 一抗
  • 描述

    小鼠单克隆抗体[M2 III-6] to MRP2
  • 宿主

    Mouse
  • 特异性

    This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Dog
  • 免疫原

    Recombinant fragment within Human MRP2 aa 1300-1550 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

  • 表位

    This antibody reacts with an internal epitope of MRP 2.
  • 常规说明

    We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 克隆

    单克隆
  • 克隆编号

    M2 III-6
  • 同种型

    IgG2a
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cancer
    • Drug resistance
    • MRP-related proteins
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • KO cell lines

    • Human ABCC2 knockout A549 cell line (ab261855)
  • KO cell lysates

    • Human ABCC2 knockout A549 cell lysate (ab261663)
  • Recombinant Protein

    • Recombinant Human MRP2 protein (ab112272)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3373于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/20 - 1/50. Predicted molecular weight: 174 kDa.

See Scheffer GL et al. 2000.

The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).
The protein is glycosylated at amino acid 7, 12 and 1011 so higher band size is expected.

说明
WB
1/20 - 1/50. Predicted molecular weight: 174 kDa.

See Scheffer GL et al. 2000.

The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).
The protein is glycosylated at amino acid 7, 12 and 1011 so higher band size is expected.

靶标

  • 功能

    Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
  • 组织特异性

    Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
  • 疾病相关

    Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
  • 序列相似性

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession Q92887 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1244 Human
    • Omim: 601107 Human
    • SwissProt: Q92887 Human
    • Unigene: 368243 Human
    • 别名

      • ABC30 antibody
      • abcC2 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
      • ATP binding cassette subfamily C member 2 antibody
      • ATP-binding cassette sub-family C member 2 antibody
      • Canalicular multidrug resistance protein antibody
      • Canalicular multispecific organic anion transporter 1 antibody
      • CMOAT antibody
      • CMOAT1 antibody
      • cMRP antibody
      • DJS antibody
      • KIAA1010 antibody
      • MRP 2 antibody
      • MRP2_HUMAN antibody
      • Multidrug resistance associated protein 2 antibody
      • Multidrug resistance-associated protein 2 antibody
      see all

    图片

    • Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)
      Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)
      All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution

      Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 174 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.

      ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)
      Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)This image is courtesy of an anonymous Abreview
      Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST

      Secondary
      HRP conjugated Donkey anti-Mouse at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 174 kDa
      Observed band size: 130-250 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes

      See Abreview

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (72)

    发表研究结果有使用 ab3373?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3373 被引用在 72 文献中.

    • Zhu Y  et al. SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by ß-catenin and Beclin1/autophagy signaling in colorectal cancer. Cell Death Dis 12:449 (2021). PubMed: 33953166
    • Kongmanas K  et al. Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy. PLoS Negl Trop Dis 14:e0008835 (2020). PubMed: 33216752
    • Po A  et al. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells. Sci Rep 10:13988 (2020). PubMed: 32814794
    • Barok M  et al. ARX788, a novel anti-HER2 antibody-drug conjugate, shows anti-tumor effects in preclinical models of trastuzumab emtansine-resistant HER2-positive breast cancer and gastric cancer. Cancer Lett 473:156-163 (2020). PubMed: 31904483
    • Subramani PA  et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 18 Abreviews or Q&A

    Western blot abreview for Anti-MRP2 antibody [M2 III-6]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    15 µg
    Specification
    Hepatocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 13 2021

    Western blot abreview for Anti-MRP2 antibody [M2 III-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer Cell line)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    25 µg
    Specification
    Cancer Cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 28 2018

    Western blot abreview for Anti-MRP2 antibody [M2 III-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    15 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    提交于 Jan 24 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MRP2 antibody [M2 III-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    提交于 Nov 17 2016

    Western blot abreview for Anti-MRP2 antibody [M2 III-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (WIF-B cells, the hepatoma-derived hybrid cell line)
    Gel Running Conditions
    Reduced Denaturing (6% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    WIF-B cells, the hepatoma-derived hybrid cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 09 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MRP2 antibody [M2 III-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2 cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    HepG2 cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    提交于 Oct 14 2016

    Question

    Product code: 3373
    Inquiry: Is it known whether the anti-MRP2 antibody (ab3373) cross reacts with other MRPs (1 up to 7) in Western Blots? Thank you

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer


    I can confirm that anti MRP2 antibody ab3373 does not cross react with other MRPs.

    Read More

    Abcam Scientific Support

    回复于 May 14 2013

    Question

    Reviewing this case, I would like to offer some suggestions to help optimise the results from these antibodies. I would also appreciate if you can confirm some further details:
    1. Please confirm the order numbers and dates of purchase.
    Order reference number: #####

    Dates: 06/08/2012
    2. Could you confirm which lysis buffer was used? I can recommend to try RIPA buffer if not already tried. This should provide a suitable protein preparation.
    The lysis buffer was a fixed kit, we bought from ThermoFischer to isolate membrane proteins and a buffer based on EDTA.
    3. We recommend samples should be reduced and denatured and run on a reducing gel in WB unless stated otherwise on the datasheet. This will ensure the protein is in the correct conformation to run at the correct molecular weight and be detected by the antibody.
    The samples were reduced and diluted with the Laemmli buffer. Up to now I never used a reducing gel for my other ABC transporters and it always worked.
    4. I can recommend to consider trying BSA rather than milk to block. Changing blocking agent can sometimes help to improve results.
    This I will try
    5. Could you confirm if the transfer to the membrane and quality of the samples been assessed using a loading control?
    A coloured marker was used, showing a positive transfer onto the blot. A loading control was not used.
    6. Are the current vials of secondary antibody and detection kit working well with other primary antibodies?
    The detection kit works with other primary antibodies, the second antibody is new and didn't worked up to now. Can you maybe suggests an other working second antibody for these antibodies, I need with FITC labelling for IHC too.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2012

    Answer

    Thank you for your reply and for kindly providing the requested details.

    I look forward to hearing from you with the results of the next experiment. i can recommend it would be beneficial to consider a reducing gel. This will ensure the protein is in the correct conformation for detection by the antibody. Although the other antibodies work well using a non reducing gel, individual antibodies detect individual epitopes and so will sometimes require individual optimization.

    I hope this will be helpful. Please do not hesitate to let me know how you get on with the reducing gel. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Oct 10 2012

    Question

    Thank you for your time and cooperation. I look forward to receiving the completed questionnaire.
    Order Details
    Antibody code:
    MRP4: ab15602, GR79982-1
    MRP5: ab 3377, GR43649-3
    MRP2: ab 3373, GR19771-1
    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background
    No signal in IHC for MRP4 and MRP5
    Lot number
    MRP4: ab15602, GR79982-1
    MRP5: ab 3377, GR43649-3
    MRP2: ab 3373, GR19771-1
    Purchase order number
    or preferably Abcam order number:
    General Information
    Antibody storage conditions (temperature/reconstitution etc)
    -20oC except MRP5, fridge
    Description of the problem (high background, wrong band size, more bands, no band etc.)
    No band

    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
    Cell extract

    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
    Lyse Buffer with EDTA + Protease inhibitor cocktail

    Amount of protein loaded
    around 20 ug
    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
    Non-reducing gel, 7.5% gel, running for 90 minutes, 150V, 80mA and 25W
    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
    Buffer = without Methanol, with glycin and SDS for 2.5h with 320mA for two gels, blocking agent is a 2% Milkpowder in PBS+Tween20
    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    MRP4: 1/50 dilution in milkpowder solution
    MRP5: 1/50 dilution in milkpowder solution
    MRP2: 1/100 dilution in milkpowder solution
    Incubation in fridge overnight, washstep 3 times for 10 minutes with PBS+Tween20
    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    Secondary antibody is from calbiochem, againt the whole IgG fraction. Produced in mouse or rat
    MRP4: 1/100 dilution in milkpowder solution
    MRP5: 1/50 dilution in milkpowder solution
    MRP2: 1/100 dilution in milkpowder solution
    Incubation 1Hour at roomtemperature, washstep 3 times for 10 minutes with PBS+Tween20
    Detection method (ECL, ECLPlus etc.)
    Peroxidase, chemilumiscence
    Positive and negative controls used (please specify)
    My positive control is the caco2 cell line, in which no detection occurs, no negative controls were used
    Optimization attempts (problem solving)
    How many times have you tried the Western?
    Two times
    Have you run a "No Primary" control?
    No
    Do you obtain the same results every time?
    Yes
    e.g. are the background bands always in the same place?
    There are no background bands
    What steps have you altered?
    Non
    Additional Notes:
    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to assess the results.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 09 2012

    Answer

    Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would like to offer some suggestions to help optimise the results from these antibodies. I would also appreciate if you can confirm some further details:

    1. Please confirm the order numbers and dates of purchase.

    2. Could you confirm which lysis buffer was used? I can recommend to try RIPA buffer if not already tried. This should provide a suitable protein preparation.

    3. We recommend samples should be reduced and denatured and run on a reducing gel in WB unless stated otherwise on the datasheet. This will ensure the protein is in the correct conformation to run at the correct molecular weight and be detected by the antibody.

    4. I can recommend to consider trying BSA rather than milk to block. Changing blocking agent can sometimes help to improve results.

    5. Could you confirm if the transfer to the membrane and quality of the samples been assessed using a loading control?

    6. Are the current vials of secondary antibody and detection kit working well with other primary antibodies?

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Abcam Scientific Support

    回复于 Oct 09 2012

    Question

    Hallo,

    I tried to localize MRP4 and MRP5 in Caco2 cells cultivated in chamber
    slides. The second antibody was a goat anti-rat FITC labelled from
    another company. Both antibodies didin't show any fluorescence. Is it maybe possible to get a small amount of specific secondary antibody, to see if the problem is maybe laying there?

    The other problem I have with the MRP2 antibody in WB. The antibody worked fine in IHC, but no signal can be detected in WB. Can a polyclonal antibody be the right solution?

    Abcam code:
    MRP4: ab15602, GR79982-1
    MRP5: ab 3377, GR43649-3
    MRP2: ab 3373, GR19771-1

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible for us to stock small sample sizes of our products to send to customers. If you are concerned about the secondary antibody, I can recommend to consider contacting the supplier of the secondary. I notice that MRP4 antibody [M4I-10] (ab15602) is a rat IgG2a, do you know that the secondary antibody will detect this isotype?

    ab3373 Anti-MRP2 antibody [M2 III-6] should be working in WB, it has been tested successfully. Particularly if it has worked in the IHC experiments which shows the antibody is active, it is unusual this is not working in WB. Again, this is a mouse IgG2a isotype. Will the secondary antibody used in WB detect this, was this the same secondary used in IHC-P?

    I would like to reassure you that all these antibodies are tested and covered by our 6 month guarantee for WB and the species that are listed on the individual datasheets. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    Thank you for your time and cooperation. I look forward to receiving the completed questionnaire.


    Order Details
    Antibody code:
    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background
    Lot number
    Purchase order number
    or preferably Abcam order number:
    General Information
    Antibody storage conditions (temperature/reconstitution etc)
    Description of the problem (high background, wrong band size, more bands, no band etc.)
    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
    Amount of protein loaded
    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    Detection method (ECL, ECLPlus etc.)
    Positive and negative controls used (please specify)
    Optimization attempts (problem solving)
    How many times have you tried the Western?
    Have you run a "No Primary" control?
    Yes No
    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?
    What steps have you altered?
    Additional Notes:
    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to assess the results.

    Read More

    Abcam Scientific Support

    回复于 Oct 05 2012

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