Anti-MRP2抗体[M2 III-6] (ab3373)
Key features and details
- Mouse monoclonal [M2 III-6] to MRP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-MRP2抗体[M2 III-6]
参阅全部 MRP2 一抗 -
描述
小鼠单克隆抗体[M2 III-6] to MRP2 -
宿主
Mouse -
特异性
This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Dog -
免疫原
Recombinant fragment within Human MRP2 aa 1300-1550 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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表位
This antibody reacts with an internal epitope of MRP 2. -
常规说明
We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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克隆
单克隆 -
克隆编号
M2 III-6 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (4) |
1/20 - 1/50. Predicted molecular weight: 174 kDa.
See Scheffer GL et al. 2000. The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013). |
说明 |
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WB
1/20 - 1/50. Predicted molecular weight: 174 kDa. See Scheffer GL et al. 2000. The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013). |
靶标
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功能
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. -
组织特异性
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. -
疾病相关
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. -
序列相似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1244 Human
- Omim: 601107 Human
- SwissProt: Q92887 Human
- Unigene: 368243 Human
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别名
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
see all
图片
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All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 174 kDaLanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373)This image is courtesy of an anonymous AbreviewAnti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST
Secondary
HRP conjugated Donkey anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 174 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (83)
ab3373 被引用在 83 文献中.
- Rose S et al. DMSO-free highly differentiated HepaRG spheroids for chronic toxicity, liver functions and genotoxicity studies. Arch Toxicol 96:243-258 (2022). PubMed: 34762139
- Seo JE et al. Evaluation of an in vitro three-dimensional HepaRG spheroid model for genotoxicity testing using the high-throughput CometChip platform. ALTEX 39:583-604 (2022). PubMed: 35791290
- Horiuchi S et al. Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation. Sci Rep 12:15192 (2022). PubMed: 36071090
- Charbonnier P et al. ATP7B-Deficient Hepatocytes Reveal the Importance of Protein Misfolding Induced at Low Copper Concentration. Cells 11:N/A (2022). PubMed: 36359796
- Balasubramaniyan N et al. miR-199a-5p inhibits the expression of ABCB11 in obstructive cholestasis. J Biol Chem 297:101400 (2021). PubMed: 34774795