重组MRP1抗体[EPR21061](ab230948)|Abcam中文官网

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Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21061] to MRP1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Carrier Free

产品名称

Anti-MRP1抗体[EPR21061]
参阅全部 MRP1 一抗
描述

兔单克隆抗体[EPR21061] to MRP1
宿主

Rabbit
经测试应用

适用于: IHC-P, WBmore details
种属反应性

与反应: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa, A549, BxPC-3, SK-OV-3 and HCT 116 whole cell lysate. IHC-P: Human colon cancer, esophagus cancer and lung cancer tissue.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR21061
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
- Anti-MRP1 antibody [EPR21061] - BSA and Azide free (ab234104)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human MRP1 protein (His tag) (ab235016)

The Abpromise guarantee

Abpromise™承诺保证使用ab230948于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Predicted molecular weight: 171 kDa.

说明
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 171 kDa.

功能

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
组织特异性

Lung, testis and peripheral blood mononuclear cells.
序列相似性

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
细胞定位

Cell membrane.
Target information above from: UniProt accession P33527 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
别名
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
- ABCC antibody
- Abcc1 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
- ATP binding cassette sub-family C member 1 antibody
- ATP binding cassette subfamily C member 1 antibody
- ATP binding cassette transporter variant ABCC1delta ex13 antibody
- ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
- ATP binding cassette transporter variant ABCC1delta ex25 antibody
- ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
- ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
- ATP-binding cassette sub-family C member 1 antibody
- DKFZp686N04233 antibody
- DKFZp781G125 antibody
- GS X antibody
- GSX antibody
- Leukotriene C(4) transporter antibody
- LTC4 transporter antibody
- MRP 1 antibody
- MRP antibody
- MRP1 antibody
- MRP1_HUMAN antibody
- Multidrug resistance associated protein 1 antibody
- Multidrug resistance protein antibody
- Multidrug resistance-associated protein 1 antibody
- Multiple drug resistance associated protein antibody
- Multiple drug resistance protein 1 antibody
see all

Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : ABCC1 knockout A549 cell lysate
Lane 3 : Wild-type HeLa cell lysate
Lane 4 : ABCC1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 171 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab230948 observed at 170 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab230948 was shown to react with MRP1 in wild-type HeLa cells in Western blot with loss of signal observed in ABCC1 knockout cell line (ABCC1 knockout cell lysate). Wild-type HeLa and ABCC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab230948 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous staining in human esophageal cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:26870278) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate
Lane 5 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 171 kDa

Exposure time: 3 minutes

Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human colon cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human colon cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-MRP1 antibody [EPR21061] (ab230948)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

Download

发表研究结果有使用 ab230948？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab230948 被引用在 2 文献中.

Ding L et al. Cisplatin resistance and malignant behaviors of lung cancer cells are promoted by circ_0002360 via targeting miR-6751-3p to regulate the expression of ZNF300. Thorac Cancer 13:986-996 (2022). PubMed: 35166026
Xu X et al. Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway. Cancer Cell Int 20:552 (2020). PubMed: 33292236

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Immunohistochemistry reagents

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (2)

客户评价及客户问答