Anti-MRF-1抗体(ab222827)
Key features and details
- Rabbit polyclonal to MRF-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MRF-1抗体 -
描述
兔多克隆抗体to MRF-1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MRF-1.
Database link: O75570 -
阳性对照
- WB: HeLa whole cell lysate. IHC-P: Human liver cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
>95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
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| WB |
1/500 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53,55 kDa).
|
| 说明 |
|---|
|
IHC-P
1/20 - 1/200. |
|
WB
1/500 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53,55 kDa). |
靶标
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功能
Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA. -
序列相似性
Belongs to the prokaryotic/mitochondrial release factor family. -
翻译后修饰
Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 9617 Human
- Omim: 604601 Human
- SwissProt: O75570 Human
- Unigene: 382176 Human
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别名
- mitochondrial antibody
- MRF-1 antibody
- MRF1 antibody
see all
图片
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Western blot - Anti-MRF-1 antibody (ab222827)Anti-MRF-1 antibody (ab222827) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 53,55 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRF-1 antibody (ab222827)Paraffin-embedded sections of human liver cancer tissue were stained for MRF-1 using ab222827 at 1/100 dilution in immunohistochemical analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab222827 尚未被引用在任何文献中。