重组Anti-Mre11抗体[EPR3471] (ab109623)
![Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.cn/ps/products/109/ab109623/Images/ab109623-122569-anti-mre11-antibody-epr3471-western-blot.jpg "Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3471] to Mre11
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Mre11抗体[EPR3471]
参阅全部 Mre11 一抗 -
描述
兔单克隆抗体[EPR3471] to Mre11 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, HT-29, Jurkat, and HeLa whole cell lysate (ab150035). Human cervical carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3471 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109623于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 81 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 81 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
疾病相关
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
序列相似性
Belongs to the MRE11/RAD32 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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数据库链接
- Entrez Gene: 4361 Human
- Entrez Gene: 17535 Mouse
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q61216 Mouse
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
see all -
别名
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
图片
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Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)All lanes : Anti-Mre11 antibody [EPR3471] (ab109623) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 81 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.cn/ps/products/109/ab109623/Images/ab109623-122568-anti-mre11-antibody-epr3471-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR3471] (ab109623)ab109623 at 1/50 dilution staining Mre11 in paraffin-embedded Human cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.cn/ps/products/109/ab109623/Images/ab109623-3-benefits-of-recombinant-antibodies.png)
Anti-Mre11 antibody [EPR3471] (ab109623)
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109623 被引用在 6 文献中.
- Feng YL et al. Proximal binding of dCas9 at a DNA double strand break stimulates homology-directed repair as a local inhibitor of classical non-homologous end joining. Nucleic Acids Res 51:2740-2758 (2023). PubMed: 36864759
- Huang M et al. PARP1 negatively regulates transcription of BLM through its interaction with HSP90AB1 in prostate cancer. J Transl Med 21:445 (2023). PubMed: 37415147
- Ye C et al. High glucose induces the proliferation of prostatic cells via downregulating MRE11. Int J Mol Med 41:3105-3114 (2018). PubMed: 29532862
- Vadnais C et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun 9:1418 (2018). PubMed: 29651020
- Tsukagoshi M et al. Overexpression of karyopherin-a2 in cholangiocarcinoma correlates with poor prognosis and gemcitabine sensitivity via nuclear translocation of DNA repair proteins. Oncotarget 8:42159-42172 (2017). PubMed: 28178675
- Altan B et al. High Expression of MRE11-RAD50-NBS1 Is Associated with Poor Prognosis and Chemoresistance in Gastric Cancer. Anticancer Res 36:5237-5247 (2016). PubMed: 27798884