为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling Chromosome Structure Telomeres

Anti-Mre11抗体[12D7] - BSA and Azide free (ab214)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (79)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

Key features and details

  • Mouse monoclonal [12D7] to Mre11 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-Fr, IHC-P, IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

你可能会感兴趣

蛋白
Product image
Recombinant Human Mre11 protein (ab114277)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一抗
Product image
Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420)

查看更多相关产品

概述

  • 产品名称

    Anti-Mre11抗体[12D7] - BSA and Azide free
    参阅全部 Mre11 一抗
  • 描述

    小鼠单克隆抗体[12D7] to Mre11 - BSA and Azide free
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt, IHC-Fr, IHC-P, IP, ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mre11 aa 150-600.

  • 阳性对照

    • WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Constituent: 100% PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    12D7
  • 骨髓瘤

    NS1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Mre11 protein (ab114277)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab214于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

For normal lymphoblastoid cell lines.

ICC/IF (2)
1/100 - 1/1000.
WB (4)
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

说明
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

For normal lymphoblastoid cell lines.

ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

靶标

  • 功能

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 疾病相关

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • 序列相似性

    Belongs to the MRE11/RAD32 family.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Target information above from: UniProt accession P49959 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4361 Human
    • Entrez Gene: 17535 Mouse
    • Entrez Gene: 64046 Rat
    • Omim: 600814 Human
    • SwissProt: P49959 Human
    • SwissProt: Q61216 Mouse
    • SwissProt: Q9JIM0 Rat
    • Unigene: 192649 Human
    • Unigene: 149071 Mouse
    • Unigene: 209040 Rat
    see all
  • 别名

    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

图片

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

  • Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

    Secondary
    Anti-mouse IgG HRP-conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

    The signal was developed with Trident ECL plus-Enhanced.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    This image was generated using the ascites version of the product.

    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

    Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
    (Panel D - negative control).

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/500 dilution

    Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
    Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This image was generated using the ascites version of the product.

    See Abreview

实验方案

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (79)

发表研究结果有使用 ab214?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab214 被引用在 79 文献中.

  • Elkholi IE  et al. Investigating the causal role of MRE11A p.E506* in breast and ovarian cancer. Sci Rep 11:2409 (2021). PubMed: 33510186
  • Na J  et al. SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells. Cell Death Dis 12:165 (2021). PubMed: 33558481
  • Hu S  et al. LINCS gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1. Int J Mol Med 47:N/A (2021). PubMed: 33693953
  • Kang JW  et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
  • Kilgas S  et al. p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation. Cell Rep 35:109153 (2021). PubMed: 34038735
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody [12D7]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (LLC-PK1 cell line)
Permeabilization
Yes - 0.5% Triton-X 100
Specification
LLC-PK1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 10 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Mre11 [12D7] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Tissue sections (Normal or gastric cancer tissues)
Specification
Normal or gastric cancer tissues
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 20 2014

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Cultured Cells (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 19 2014

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (Hela cell line)
Specification
Hela cell line
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 18 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 [12D7] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cell (Human colon cancer cell lines)
Specification
Human colon cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 15 2013

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
Specification
Mouse breast cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 15 2013

Western blot abreview for Anti-Mre11 antibody [12D7]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
15 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 08 2011

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (human cancer cells (colon, bladder))
Loading amount
20 µg
Specification
human cancer cells (colon, bladder)
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 12 2011

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 17 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mre11 antibody [12D7]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Bronchus)
Specification
Normal Bronchus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有