MRC2/ENDO180重组抗体[EPR29048-78]_MRC2/ENDO180抗体(ab317749)

产品名称

Anti-MRC2/ENDO180抗体[EPR29048-78] - BSA and Azide free
参阅全部 MRC2/ENDO180 一抗
描述

兔单克隆抗体[EPR29048-78] to MRC2/ENDO180 - BSA and Azide free
宿主

Rabbit
经测试应用

适用于: IHC-P, IP, WBmore details
不适用于: Flow Cyt or ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Saos-2, MG-63, U-2 OS, A-172, Human liver cancer, Human tonsil, Human breast, Human kidney, Mouse breast, Mouse spleen, Mouse lung, Rat kidney and Rat spleen lysates. IHC-P: Human kidney, Human tonsil, Human breast carcinoma, Rat kidney, Mouse kidney and Mouse breast carcinoma. IP: A-172 cell.
常规说明
ab317749 is the carrier-free version of ab317748
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C.
存储溶液

pH: 7.2
Constituent: 100% PBS
无载体

是
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR29048-78
同种型

IgG
研究领域

Alternative Versions
- Anti-MRC2/ENDO180 antibody [EPR29048-78] (ab317748)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Abpromise™承诺保证使用ab317749于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight: 167 kDa.

说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 167 kDa.

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

功能

May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).
组织特异性

Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.
序列相似性

Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
结构域

C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
Fibronectin type-II domain mediates collagen-binding.
Ricin B-type lectin domain contacts with the second C-type lectin domain.
翻译后修饰

N-glycosylated.
细胞定位

Membrane.
Target information above from: UniProt accession Q9UBG0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 9902 Human
- Entrez Gene: 17534 Mouse
- Entrez Gene: 498011 Rat
- Omim: 612264 Human
- SwissProt: Q9UBG0 Human
- SwissProt: Q64449 Mouse
- SwissProt: Q4TU93 Rat
- Unigene: 7835 Human
- Unigene: 235616 Mouse
- Unigene: 213511 Rat
see all
别名
- C-type lectin domain family 13 member E antibody
- C-type mannose receptor 2 antibody
- CD 280 antibody
- CD280 antibody
- CD280 antigen antibody
- CLEC 13E antibody
- CLEC13E antibody
- ENDO 180 antibody
- ENDO180 antibody
- Endocytic receptor (macrophage mannose receptor family) antibody
- Endocytic receptor 180 antibody
- FLJ35911 antibody
- KIAA0709 antibody
- Macrophage mannose receptor 2 antibody
- Mannose receptor C type 2 antibody
- MRC 2 antibody
- MRC2 antibody
- MRC2_HUMAN antibody
- UPAR-associated protein antibody
- UPARAP antibody
- Urokinase plasminogen activator receptor associated protein antibody
- Urokinase receptor associated protein antibody
- Urokinase receptor-associated protein antibody
- Urokinase-type plasminogen activator receptor-associated protein antibody
see all

Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

All lanes : Anti-MRC2/ENDO180 antibody [EPR29048-78] (ab317748) at 1/1000 dilution

Lane 1 : Mouse breast tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat kidney tissue lysate
Lane 6 : Rat spleen tissue lysate
Lane 7 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 167 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression tissue: brain (PMID: 8702911).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-3: 180 seconds; Lane 4: 15 seconds; Lanes 5-7: 59 seconds.
Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

All lanes : Anti-MRC2/ENDO180 antibody [EPR29048-78] (ab317748) at 1/1000 dilution

Lane 1 : Human liver cancer tissue lysate
Lane 2 : Human tonsil tissue lysate
Lane 3 : Human breast tissue lysate
Lane 4 : Human kidney tissue lysate
Lane 5 : Human brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 167 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression tissue: brain (PMID: 8702911).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

All lanes : Anti-MRC2/ENDO180 antibody [EPR29048-78] (ab317748) at 1/1000 dilution

Lane 1 : Saos-2 (human osteosarcoma epithelial cell) whole cell lysate
Lane 2 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 6 : A-172 (human brain glioblastoma cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 167 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: MCF7, HepG2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-4: 26 seconds; Lanes 5-6: 8 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stromal cells of human kidney (PMID: 11156692).
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil (PMID: 8702911).
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of human breast carcinoma (PMID 26316068).
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of mouse kidney.
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of rat kidney.
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of mouse breast carcinoma.
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue: weak staining on endothelium cells of human cerebrum (PMID: 8702911).
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on endothelium cells of mouse cerebrum.
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MRC2/ENDO180 with ab317748 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: weak staining on endothelium cells of rat cerebrum.
The section was incubated with ab317748 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

This data was developed using ab317748, the same antibody clone in a different buffer formulation.
MRC2/ENDO180 was immunoprecipitated from 0.35 mg A-172 (human brain glioblastoma cell ) whole cell lysate with ab317748 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317748 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A-172 (human brain glioblastoma cell ) whole cell lysate
Lane 2: ab317748 IP in A-172 (human brain glioblastoma cell ) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317748 in A-172 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds..
Anti-MRC2/ENDO180 antibody [EPR29048-78] - BSA and Azide free (ab317749)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

无载体

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

Datasheet download

Certificate of Compliance

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