重组Anti-MRC2/ENDO180抗体[EPR29048-48] (ab317323)
![Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-539769-anti-mrc2endo180-antibody-epr29048-48-western-blot-saos-2-human-osteosarcoma-epithelial-cell-whole-cell-lysate20-ug.jpg "Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR29048-48] to MRC2/ENDO180
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MRC2/ENDO180抗体[EPR29048-48]
参阅全部 MRC2/ENDO180 一抗 -
描述
兔单克隆抗体[EPR29048-48] to MRC2/ENDO180 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Saos-2, MG-63, U-2 OS, A-172, Human liver cancer, Human tonsil and Human brain lysates. IHC-P: Human kidney, Human tonsil and Human breast carcinoma tissues. ICC: MG 63 cells. Flow Cyt: MG-63 cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR29048-48 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/500.
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/1000. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
1/500. |
|
ICC/IF
1/100. |
靶标
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功能
May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). -
组织特异性
Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney. -
序列相似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
结构域
C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
Fibronectin type-II domain mediates collagen-binding.
Ricin B-type lectin domain contacts with the second C-type lectin domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9902 Human
- Omim: 612264 Human
- SwissProt: Q9UBG0 Human
- Unigene: 7835 Human
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别名
- C-type lectin domain family 13 member E antibody
- C-type mannose receptor 2 antibody
- CD 280 antibody
see all
图片
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Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)All lanes : Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323) at 1/1000 dilution
Lane 1 : Saos-2 (human osteosarcoma epithelial cell) whole cell lysate
Lane 2 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 6 : A-172 (human brain glioblastoma cell ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 6 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MCF7, HepG2.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-539770-anti-mrc2endo180-antibody-epr29048-48-western-blot-human-liver-cancer-tissue-lysate20-ug.jpg)
Western blot - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)All lanes : Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323) at 1/1000 dilution
Lane 1 : Human liver cancer tissue lysate
Lane 2 : Human tonsil tissue lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: human brain (PMID: 8702911).
The identity of the lower MW band at approximately 65 kDa (in lane 3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 103 seconds; Lanes 2-3: 180 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-2-anti-mrc2endo180-antibody-epr29048-48-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in endothelial cells of human kidney
(PMID: 11156692).
The section was incubated with ab317323 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-3-anti-mrc2endo180-antibody-epr29048-48-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in macrophages of human tonsil
(PMID: 8702911).
The section was incubated with ab317323 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-4-anti-mrc2endo180-antibody-epr29048-48-immunohistochemistry-human-breast-carcino.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal cells of human breast carcinoma
(PMID 26316068).
The section was incubated with ab317323 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-5-anti-mrc2endo180-antibody-epr29048-48-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MRC2/ENDO180 with ab317323 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on human cerebrum
(PMID: 8702911).
The section was incubated with ab317323 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-1-anti-mrc2endo180-antibody-epr29048-48-immunocytochemistry-mg-63.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MG 63 (human osteosarcoma fibroblast) cells labelling MRC2/ENDO180 with ab317323 at 1/100 (4.98 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in MG 63 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: MCF7 (PMID: 17974964).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Flow Cytometry - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-6-anti-mrc2endo180-antibody-epr29048-48-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / MG-63(human osteosarcoma fibroblast, Right) cells labelling MRC2/ENDO180 with ab317323 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: MCF7
Gated on viable cell. -
![Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)](https://www.abcam.cn/ps/products/317/ab317323/Images/ab317323-7-benefitsofrecombinantantibodies.jpg)
Anti-MRC2/ENDO180 antibody [EPR29048-48] (ab317323)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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