AB176570

Anti-MRas 抗体 [EPR12457]

Anti-MRas antibody [EPR12457]

RabMAb
Recombinant
KO Validated
20ul selling size
了解详情

Be the first to review this product! Submit a review

(5 Publications)

Anti-MRas antibody [EPR12457] (ab176570) is a rabbit monoclonal antibody detecting MRas in Western Blot, IP. Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

查看别名

RRAS3, MRAS, Ras-related protein M-Ras, Ras-related protein R-Ras3

4 Images

Lab

Immunoprecipitation - Anti-MRas antibody [EPR12457] (AB176570)

Purified ab176570 at 1/20 dilution (1μg) immunoprecipitating MRas in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab176570 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab176570 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 24 kDa

All lanes:

Immunoprecipitation - Anti-MRas antibody [EPR12457] (ab176570)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-MRas antibody [EPR12457] (AB176570)

All lanes:

Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

All lanes:

Human brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 24 kDa

false

Lab

Western blot - Anti-MRas antibody [EPR12457] (AB176570)

Lanes 1-3 : Merged signal (red and green). Green - ab176570 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265510 (knockout cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MRAS knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MRAS knockout HeLa cell line (<a href='/products/cell-lines/human-mras-knockout-hela-cell-line-ab265510'>ab265510</a>)

Lane 3:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Unknown

Western blot - Anti-MRas antibody [EPR12457] (AB176570)

All lanes:

Western blot - Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-MRas antibody [EPR12457] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12457

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

What is this antibody validated in?
Anti-MRas antibody [EPR12457] (ab176570) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse samples.

What is the molecular weight of MRas?
Anti-MRas [EPR12457] (ab176570) specifically detects a band for MRas (UniProt: O14807) at a molecular weight of 24kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-MRas antibody [EPR12457] (ab176570) has been confirmed by Western blot testing in MRAS Knockout HeLa cell line, ab265510.

Other related products
We have a range of other formats of antibody clone [EPR12457] also available for your convenience: ab176570, Carrier free - ab240194

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MRas is a Ras-related GTP-binding protein often referred to as Ras-related protein M-Ras which belongs to the Ras superfamily of small GTPases. It has a molecular mass of about 24 kDa. MRas is expressed widely but shows higher levels in the brain and heart tissues. It functions by cycling between inactive GDP-bound and active GTP-bound states an action that modulates signal transduction pathways within cells. The process is imperative for its role in regulating cell dynamics.

Biological function summary

MRas plays a role in several cellular processes including cell growth differentiation and survival. It does not form part of larger complexes but interacts with various cytoplasmic and membrane proteins to propagate signals that control these cellular functions. Studies illustrate MRas's involvement in the regulation of cytoskeleton restructuring and cellular morphology further indicating its association with cell motility.

Pathways

MRas participates in the MAPK/ERK signaling pathway which is important for mediating cellular responses to growth signals. It associates with the Raf/MEK/ERK pathway components functioning upstream to influence these pathways. MRas's activity connects to other proteins like ERK1/2 allowing it to regulate gene expression involved in cell proliferation and survival.

MRas has been linked to cardiovascular diseases and certain types of cancer. Its role in cardiomyopathy stems from its involvement in cardiac cell signal transduction pathways. Additionally MRas activity can influence oncogenic pathways which relates to tumors driven by aberrant Ras signaling. The MRas interaction with Raf in particular is notable in its contribution to cancer pathology.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 16630891, PubMed : 28289718, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670).

See full target information MRAS

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1054 PubMed41053757

2025

ATP5F1A deficiency causes developmental delay and motor dysfunction in humans and zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Xian,Qing Luo,Weiping Li,Lin Zou,Jinbo Liu

Nature cancer 5:1352-1370 PubMed39103541

2024

Co-targeting SOS1 enhances the antitumor effects of KRAS inhibitors by addressing intrinsic and acquired resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Venu Thatikonda,Hengyu Lyu,Sabine Jurado,Kaja Kostyrko,Christopher A Bristow,Christoph Albrecht,Donat Alpar,Heribert Arnhof,Oliver Bergner,Karin Bosch,Ningping Feng,Sisi Gao,Daniel Gerlach,Michael Gmachl,Melanie Hinkel,Simone Lieb,Astrid Jeschko,Annette A Machado,Thomas Madensky,Ethan D Marszalek,Mikhila Mahendra,Gabriella Melo-Zainzinger,Jessica M Molkentine,Philipp A Jaeger,David H Peng,Robyn L Schenk,Alexey Sorokin,Sandra Strauss,Francesca Trapani,Scott Kopetz,Christopher P Vellano,Mark Petronczki,Norbert Kraut,Timothy P Heffernan,Joseph R Marszalek,Mark Pearson,Irene C Waizenegger,Marco H Hofmann

Cell death & disease 14:556 PubMed37626065

2023

LZTR1 deficiency exerts high metastatic potential by enhancing sensitivity to EMT induction and controlling KLHL12-mediated collagen secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Taiki Abe,Shin-Ichiro Kanno,Tetsuya Niihori,Miho Terao,Shuji Takada,Yoko Aoki

The Journal of experimental medicine 220: PubMed36820830

2023

Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Mitchell J Geer,Xinyi Guo,Igor Dolgalev,Neville E Sanjana,Benjamin G Neel

Cancer discovery 12:2434-2453 PubMed35904492

2022

Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Rahul S Vedula,Antonio Cuevas-Navarro,Bin Lu,Simon J Hogg,Eric Wang,Salima Benbarche,Katherine Knorr,Won Jun Kim,Robert F Stanley,Hana Cho,Caroline Erickson,Michael Singer,Dan Cui,Steven Tittley,Benjamin H Durham,Tatiana S Pavletich,Elise Fiala,Michael F Walsh,Daichi Inoue,Sebastien Monette,Justin Taylor,Neal Rosen,Frank McCormick,R Coleman Lindsley,Pau Castel,Omar Abdel-Wahab

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRas 抗体 [EPR12457]