Anti-MR1抗体(ab229987)
Key features and details
- Rabbit polyclonal to MR1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MR1抗体
参阅全部 MR1 一抗 -
描述
兔多克隆抗体to MR1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human MR1 aa 150-253.
Database link: Q95460 -
阳性对照
- WB: THP-1 and K562 whole cell lysates; Mouse brain lysate. IHC-P: Human liver and kidney tissues. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229987于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/50 - 1/200.
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说明 |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
结构域
The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression. The alpha-3 region mediates interaction with B2M (PubMed:23051753).
The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted; Cell membrane. Endoplasmic reticulum and Cell membrane. Endoplasmic reticulum membrane. The larger proportion remains in the ER in an immature state. The subset that reach cell surface does it through a B2M-independent pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 3140 Human
- Entrez Gene: 15064 Mouse
- Omim: 600764 Human
- SwissProt: Q95460 Human
- SwissProt: O19478 Mouse
- SwissProt: Q8HWB0 Mouse
- Unigene: 13500 Human
- Unigene: 7580 Mouse
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别名
- Class I histocompatibility antigen like protein antibody
- Class I histocompatibility antigen-like protein antibody
- HLALS antibody
see all
图片
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Paraffin-embedded human kidney tissue stained for MR1 using ab229987 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-MR1 antibody (ab229987) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : Mouse brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa -
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for MR1 using ab229987at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human liver tissue stained for MR1 using ab229987 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab229987 被引用在 1 文献中.
- Cao X et al. Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening. Front Vet Sci 7:570593 (2020). PubMed: 33240950