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AB251741

Anti-MPRIP抗体

Anti-MPRIP antibody

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(1 Publication)

Rabbit Polyclonal MPRIP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus.

查看别名

KIAA0864, MRIP, RHOIP3, MPRIP, Myosin phosphatase Rho-interacting protein, M-RIP, Rho-interacting protein 3, p116Rip, RIP3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)

Immunohistochemical analysis of human cerebral cortex tissue labeling MPRIP with ab251741 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)

Immunohistochemical analysis of human prostate tissue labeling MPRIP with ab251741 at a 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MPRIP antibody (AB251741)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MPRIP antibody (AB251741)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MPRIP (green) using ab251741 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)

Immunohistochemical analysis of human Fallopian tube tissue labeling MPRIP with ab251741 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPRIP antibody (AB251741)

Immunohistochemical analysis of human liver tissue labeling MPRIP with ab251741 at a 1/200 dilution.

Western blot - Anti-MPRIP antibody (AB251741)
  • WB

Unknown

Western blot - Anti-MPRIP antibody (AB251741)

All lanes:

Western blot - Anti-MPRIP antibody (ab251741) at 0.4 µg/mL

All lanes:

PC-3 (human prostate adenocarcinoma cell line line) whole cell lysate

Predicted band size: 117 kDa

false

Western blot - Anti-MPRIP antibody (AB251741)
  • WB

Unknown

Western blot - Anti-MPRIP antibody (AB251741)

All lanes:

Western blot - Anti-MPRIP antibody (ab251741) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 117 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human MPRIP aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6WCQ1

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MPRIP also known as Myosin Phosphatase Rho Interacting Protein or p116Rip is an important player in the regulation of the actin cytoskeleton. This protein weighs approximately 116 kDa and is expressed in a variety of tissues including the brain heart and testis. It interacts with the regulatory subunit of myosin phosphatase influencing actin filament dynamics by modulating the interaction between actin and myosin.
Biological function summary

MPRIP serves a critical role in the modulation of actin cytoskeleton rearrangement. It is part of a multi-protein complex that includes myosin phosphatase which is essential for dephosphorylation of myosin light chains. By binding directly to the myosin phosphatase complex MPRIP ensures proper regulation of actin-myosin interactions which are integral to cellular processes such as migration division and shape maintenance.

Pathways

MPRIP plays an important role within the RhoA signaling pathway and the regulation of cytoskeletal rearrangements. It facilitates the interaction between RhoA and myosin phosphatase leading to the deactivation of contractility. Another important protein involved is ROCK as MPRIP counteracts ROCK-induced phosphorylation of myosin light chain therefore influencing cell contractility and motility.

MPRIP dysregulation has implications in cardiovascular diseases and cancer. Its connection with proteins like ROCK and RhoA highlights its role in these pathologies. In cardiovascular diseases improper MPRIP function can lead to aberrant vascular smooth muscle contraction while in cancer altered MPRIP activity may contribute to changes in cell migration and invasion affecting cancer progression and metastasis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
See full target information MPRIP
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 217: PubMed32453420

2020

Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxin Zhou,Meihua Lin,Xing Feng,Fei Ma,Yuekun Zhu,Xing Liu,Chao Qu,Hong Sui,Bei Sun,Anlong Zhu,Heng Zhang,He Huang,Zhi Gao,Yongxiang Zhao,Jiangyun Sun,Yuxian Bai,Junfei Jin,Xuehui Hong,Chang Zou,Zhiyong Zhang
View all publications
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