Anti-MPP1抗体(ab122165)
Key features and details
- Rabbit polyclonal to MPP1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MPP1抗体 -
描述
兔多克隆抗体to MPP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MPP1 aa 663-811.
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阳性对照
- Human duodenum tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122165于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plus-end-directed motor enzyme that is required for completion of cytokinesis. -
组织特异性
Brain, ovary, kidney and testis. -
序列相似性
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules. A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase. During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone. At anaphase it is concentrated in the mid-body. - Information by UniProt
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数据库链接
- Entrez Gene: 9585 Human
- Omim: 605498 Human
- SwissProt: Q96Q89 Human
- Unigene: 240 Human
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别名
- Cancer/testis antigen 90 antibody
- CT90 antibody
- KI20B_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MPP1 antibody (ab122165)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122165 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP1 antibody (ab122165)ab122165 at 1/75 dilution staining MPP1 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122165 被引用在 2 文献中.
- Chen J et al. KIF20B Promotes Cell Proliferation and May Be a Potential Therapeutic Target in Pancreatic Cancer. J Oncol 2021:5572402 (2021). PubMed: 34539784
- Liu X et al. Inhibition of kinesin family member 20B sensitizes hepatocellular carcinoma cell to microtubule-targeting agents by blocking cytokinesis. Cancer Sci 109:3450-3460 (2018). PubMed: 30191636