Anti-Mouse IgG2b 抗体 [RM108]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling HLA-DOB with ab312858 at 1/20000 (0.053 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human skeletal muscle. The section was incubated with ab312858 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA-DOB with ab312858 at 1/20000 (0.053 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab312858 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Mouse IgG2b antibody [RM108] (AB190482)

ab190482 reacted to non-reduced IgG2b (~150 kDa) much stronger than the reduced γ2b(~50 kDa).

All lanes:

Western blot - Anti-Mouse IgG2b antibody [RM108] (ab190482) at 0.2 µg/mL

Lane 1:

Mouse IgG2b, non-reduced at 0.02 µg

Lane 2:

Mouse IgG2b, reduced at 0.02 µg

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