小鼠IgG2a，Kappa单克隆抗体[B12/8] -同型对照(ab91361)
![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)](https://www.abcam.cn/ps/products/91/ab91361/Images/ab91361-279920-mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-flow-cytometry.jpg "Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)")
Related conjugates and formulations
概述
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产品名称
小鼠IgG2a，Kappa单克隆抗体[B12/8] -同型对照
参阅全部 Mouse 亚类对照 -
经测试应用
适用于: Flow Cytmore details -
免疫原
Synthetic peptide. Hapten from sequence of plant origin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from supernatant from an in vitro culture. -
克隆
单克隆 -
克隆编号
B12/8 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
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别名
- Mouse Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91361于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab91361 is a negative isotype control |
| 说明 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control |
图片
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Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg.
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![Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)](https://www.abcam.cn/ps/products/91/ab91361/Images/ab91361-106905-mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab91361 被引用在 9 文献中.
- Giadans CG et al. Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same. J Viral Hepat 29:124-134 (2022). PubMed: 34820942
- Mahajan AS et al. Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts. Nutrients 13:N/A (2021). PubMed: 34836359
- Benz PM et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
- Chen X et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
- Rios DA et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). PubMed: 29038590
- Khan A et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). PubMed: 29118429
- Abo Al Hayja M et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
- Jun E et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). PubMed: 25705892
- Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . PubMed: 24573683