AB91361

Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control

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(9 Publications)

Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control Suitable for Flow Cyt and reacts with samples. Cited in 9 publications.

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Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)

Flow Cyt

Supplier Data

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)

Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg.

Flow Cyt

Unknown

Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (AB91361)

Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

不同偶联物与剂型 (1)

660 APC

APC Mouse IgG2a [B12/8] - Isotype Control

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

B12/8

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

应用

Flow Cyt

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>ab91361 is a negative isotype control</p>" } } }

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产品详情

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from supernatant from an in vitro culture.

存储溶液

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of viral hepatitis 29:124-134 PubMed34820942

2021

Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Graciela Giadans,Daniela Alejandra Ríos,Beatriz Ameigeiras,Leila Haddad,Elena Noemí De Matteo,Pamela Valva,María Victoria Preciado

Nutrients 13: PubMed34836359

2021

Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Avinash S Mahajan,Venkata S Arikatla,Anita Thyagarajan,Tetyana Zhelay,Ravi P Sahu,Michael G Kemp,Dan F Spandau,Jeffrey B Travers

Acta physiologica (Oxford, England) 228:e13325 PubMed31162891

2019

AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting.

Applications

Unspecified application

Species

Unspecified reactive species

Peter M Benz,Yindi Ding,Heike Stingl,Annemarieke E Loot,Joana Zink,Ilka Wittig,Rüdiger Popp,Ingrid Fleming

Free radical biology & medicine 139:80-91 PubMed31078730

2019

Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8 T cells activation via JAK-STAT pathway in vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Xuguang Chen,Weinan Guo,Yuqian Chang,Jiaxi Chen,Pan Kang,Xiuli Yi,Tingting Cui,Sen Guo,Qian Xiao,Zhe Jian,Kai Li,Tianwen Gao,Shuli Li,Ling Liu,Chunying Li

Scientific reports 7:15010 PubMed29118429

2017

Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Khan,Lovepreet Mann,Ramesha Papanna,Mi-Ae Lyu,Christopher R Singh,Scott Olson,N Tony Eissa,Jeffrey Cirillo,Gobardhan Das,Robert L Hunter,Chinnaswamy Jagannath

Scientific reports 7:13283 PubMed29038590

2017

Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Alejandra Rios,Pamela Valva,Paola Cecilia Casciato,Silvia Frias,María Soledad Caldirola,María Isabel Gaillard,Liliana Bezrodnik,Juan Bandi,Omar Galdame,Beatriz Ameigeiras,Diana Krasniansky,Carlos Brodersen,Eduardo Mullen,Elena Noemí De Matteo,María Victoria Preciado

Journal of inflammation (London, England) 12:28 PubMed25969669

2015

Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients.

Applications

Flow Cyt

Species

Human

Muntasir Abo Al Hayja,Anders Eklund,Johan Grunewald,Jan Wahlström

PloS one 10:e0118310 PubMed25705892

2015

Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Eunsung Jun,Soyoun Kim,Jong-Ho Kim,Kiweon Cha,In-Seop So,Hye-Nam Son,Byung-Heon Lee,Kwangmeyung Kim,Ick Chan Kwon,Sang Yoon Kim,In-San Kim

The Journal of biological chemistry 289:11132-42 PubMed24573683

2014

Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.

Applications

Flow Cyt

Species

Unspecified reactive species

Yuko Tashima,Pamela Stanley

View all publications

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mouse-igg-isotype-control-ab37355

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Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control