Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control

• ChIP

Lab

ChIP - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

Chromatin was prepared from HeLa cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab5408 (red), or 5 µg of Mouse IgG1 ab18443 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.

• IP

Lab

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

Hsp27 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 μg with ab2790 1/1000 dilution (0.45 μg/ml). Western blot was performed on the immunoprecipitate using ab2790 at 1/1000 dilution. Capture antibody, 1/30 dilution (2μg in 0.35mg lysates).

Mouse monoclonal IgG1, kappa (ab18443) Isotype Control was used instead of ab2790 in HeLa whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Secondary antibody (ab131368), mouse IgG for IP (HRP) used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Hsp27 antibody [G3.1] (<a href='/products/primary-antibodies/hsp27-antibody-g31-ab2790'>ab2790</a>) at 1/1000 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell ) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Observed band size: 27 kDa

false

Exposure time: 7s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

CP2c was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308611 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution. Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 2 : ab308611 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab308611 in K-562 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-CP2c antibody [14/LSF] (<a href='/products/primary-antibodies/cp2c-antibody-14-lsf-ab308611'>ab308611</a>) at 1/30 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

p53 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab308609 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308609 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : HEK-293 whole cell lysate Lane 2 : ab308609 IP in HEK-293 whole cell lysate Lane 3 : Mouse IgG1 monoclonal isotype control (ab18443) instead of ab308609 in HEK-293 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 23 seconds. The binding pattern observed is consistent with what has been described in the literature (PMID : 31045216; PMID : 16131611).

All lanes:

Immunoprecipitation - Anti-p53 antibody [G59-12] (<a href='/products/primary-antibodies/p53-antibody-g59-12-ab308609'>ab308609</a>) at 1/1000 dilution

All lanes:

HEK-293 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

false

• IP

Lab

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

Histone H2B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab52484 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab52484 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.  Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate  10 ug Lane 2 : ab52484 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab52484 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (<a href='/products/primary-antibodies/histone-h2b-antibody-mabcam-52484-chip-grade-ab52484'>ab52484</a>) at 1/30 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

CP2c was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate 10 ug with ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308611 at 1/1000 dilution. Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) was used at 1/10000 dilution. Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate Lane 2 : ab308611 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab308611 in Hepa1-6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 59 seconds

All lanes:

Immunoprecipitation - Anti-CP2c antibody [14/LSF] (<a href='/products/primary-antibodies/cp2c-antibody-14-lsf-ab308611'>ab308611</a>) at 1/30 dilution

All lanes:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 59s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

CP2c was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 ug with ab308611 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308611 at 1/1000 dilution. Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) was used at 1/10000 dilution. Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate Lane 2 : ab308611 IP in C6 (rat glial tumor glial cell) whole cell lysate Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab308611 in C6 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 59 seconds

All lanes:

Immunoprecipitation - Anti-CP2c antibody [14/LSF] (<a href='/products/primary-antibodies/cp2c-antibody-14-lsf-ab308611'>ab308611</a>) at 1/30 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 59s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat testis tissue lysate
Lane 2 : ab317835 IP in Rat testis tissue lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysate

All lanes:

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (<a href='/products/primary-antibodies/sh3gl1-endophilin-a2-antibody-k51-1-ab317835'>ab317835</a>) at 1/30 dilution

All lanes:

Rat testis tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

SHP2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg with ab290646 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290646 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg Lane 2 : ab290646 IP in NIH/3T3 whole cell lysate Lane 3 : Mouse IgG1 monoclonal (ab18443) instead of ab290646 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

Lane 1:

Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-SHP2 antibody [79/PTP1D/SHP2] (<a href='/products/primary-antibodies/shp2-antibody-79-ptp1d-shp2-ab290646'>ab290646</a>) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Predicted band size: 68 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

Histone H2B was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab52484 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab52484 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution.  Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg Lane 2 : ab52484 IP in NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 μg Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab52484 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (<a href='/products/primary-antibodies/histone-h2b-antibody-mabcam-52484-chip-grade-ab52484'>ab52484</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

• IP

Supplier Data

Immunoprecipitation - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

Influenza A virus PB1 protein was immunoprecipitated from 0.35 mg 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate with ab324448 at 1/50 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324448 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Influenza A virus PB1 protein antibody [F5-19] (<a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a>) at 1/1000 dilution

Lane 1:

(Input) 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a> at 1/50 IP in 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Lane 3:

Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443) instead of <a href='/products/primary-antibodies/influenza-a-virus-pb1-protein-antibody-f5-19-ab324448'>ab324448</a> in 293T cells transfected with a influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 8s