小鼠IgG1，kappa单克隆抗体[15-6E10A7] -同型对照(ab170190)
![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-171551-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-flow-cytometry.jpg "Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
概述
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产品名称
小鼠IgG1，kappa单克隆抗体[15-6E10A7] -同型对照
参阅全部 Mouse 亚类对照 -
特异性
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
经测试应用
适用于: Flow Cyt, ELISA, IHC-P, ICC/IFmore details -
免疫原
Recombinant full length protein. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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常规说明
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
15-6E10A7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
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别名
- Mouse Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
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| Flow Cyt |
Use a concentration of 1 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 26627833
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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Flow Cyt
Use a concentration of 1 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. PubMed: 26627833 |
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ICC/IF
Use a concentration of 1 µg/ml. |
图片
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Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-171550-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.cn/ps/products/170/ab170190/Images/ab170190-169713-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-elisa.jpg)
ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab170190 被引用在 32 文献中.
- Jin Z & Liu Y The m6A reader YTHDC1-mediated lncRNA CTBP1-AS2 m6A modification accelerates cholangiocarcinoma progression. Heliyon 9:e19816 (2023). PubMed: 37809459
- Min Y et al. Cross species systems biology discovers glial DDR2, STOM, and KANK2 as therapeutic targets in progressive supranuclear palsy. Nat Commun 14:6801 (2023). PubMed: 37919278
- Peniche Silva CJ et al. Enthesis: not the same in each localisation - a molecular, histological and biomechanical study. Eur Cell Mater 44:43-55 (2022). PubMed: 35976149
- Yan B et al. SIRT3-Mediated CypD-K166 Deacetylation Alleviates Neuropathic Pain by Improving Mitochondrial Dysfunction and Inhibiting Oxidative Stress. Oxid Med Cell Longev 2022:4722647 (2022). PubMed: 36092157
- Jiang R et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). PubMed: 35396379