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Immunology Immunoglobulins Heavy Chain IgE

抗小鼠IgE抗体(ab19967)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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Key features and details

  • Goat polyclonal to Mouse IgE
  • Suitable for: Immunoelectrophoresis, ELISA
  • Reacts with: Mouse
  • Isotype: IgG

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概述

  • 产品名称

    抗小鼠IgE抗体
  • 描述

    山羊多克隆抗体to小鼠IgE
  • 宿主

    Goat
  • 特异性

    Reacts only with IgE by IEP, gel diffusion and ELISA.
  • 经测试应用

    适用于: Immunoelectrophoresis, ELISAmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Full length protein corresponding to Mouse Mouse IgE. Native protein

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Immunoaffinity purified off an immunoabsorbant containing a mouse IgE protein different from that used for immunization.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgE

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab19967于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunoelectrophoresis
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
说明
Immunoelectrophoresis
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

靶标

  • 相关性

    IgE mediates the immune responses in the pathogenesis of allergic asthmas. This has been shown in the successful use of recombinant 'humanised' monoclonal antibodies against IgE in the treatment of moderate-to-severe allergic asthma where the antibody forms complexes with free IgE and blocks its interaction with mast cells and basophils.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab19967?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab19967 被引用在 2 文献中.

  • Pei Y  et al. Studies on HBcAg-rBlo t 5-21 Fusion Protein Vaccine That Alleviates Blomia tropicalis Airway Inflammation. J Inflamm Res 15:6343-6355 (2022). PubMed: 36424919
  • Meixiong J  et al. Activation of Mast-Cell-Expressed Mas-Related G-Protein-Coupled Receptors Drives Non-histaminergic Itch. Immunity 50:1163-1171.e5 (2019). PubMed: 31027996

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear technical support team: The customer raises an enquiry about ab19967(Mouse IgE antibody).He would like to perform IHC with ab19967? He is very interested in abcam antibodies. Would you please help her to solve the problem? Thanks for your kindly help. Best regards

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Abcam community

Verified customer

Asked on Oct 10 2011

Answer

Thank you for contacting Abcam about ab19967. It has not been tested in IHC and so we cannot guarantee that it will work in this particular application. If you wish to take advantage of our testing discount program, then I would gladly send you the information on that program. If there is anything else I can help you with, please do hesitate to contact me.

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Abcam Scientific Support

回复于 Oct 10 2011

Question

How sensitive is this antibody in ELISA? Does it have a very high affinity for the antigen (i.e. could it be used to capture mouse IgE from solution)?

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Abcam community

Verified customer

Asked on Oct 06 2011

Answer

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding ab19967. The affinity constant and the sensitivity of this IgE antibody have not yet been determined. However, this antibody has been used to capture IgE from mouse sera so we believe it has a high affinity for the IgE. The suggested starting concentration is 1.0 - 5.0 ug/mL.   I hope this information is useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Abcam Scientific Support

回复于 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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