重组Anti-Moesin (phospho T558)抗体[EPR2371(2)] (ab177943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2371(2)] to Moesin (phospho T558)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Moesin (phospho T558)抗体[EPR2371(2)]
参阅全部 Moesin 一抗 -
描述
兔单克隆抗体[EPR2371(2)] to Moesin (phospho T558) -
宿主
Rabbit -
特异性
This antibody detects Moesin phosphorylated at threonine 558, Ezrin phosphorylated at threonine 567 and Radixin phosphorylated at threonine 564.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Calyculin treated 293T cell lysate; Human gastric adenocarcinoma and prostate tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2371(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 75, 80 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
组织特异性
In all tissues and cultured cells studied. -
序列相似性
Contains 1 FERM domain. -
翻译后修饰
Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
细胞定位
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all -
别名
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
图片
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All lanes : Anti-Moesin (phospho T558) antibody [EPR2371(2)] (ab177943) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : 293T cell lysate, treated with Calyculin
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Moesin with ab177943 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Moesin with ab177943 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab177943 被引用在 9 文献中.
- Ito Y et al. Moesin affects the plasma membrane expression and the immune checkpoint function of CD47 in human ovarian clear cell carcinoma. J Reprod Immunol 161:104185 (2024). PubMed: 38141516
- Doukuni R et al. Moesin Serves as Scaffold Protein for PD-L1 in Human Uterine Cervical Squamous Carcinoma Cells. J Clin Med 11:N/A (2022). PubMed: 35807113
- Luo S et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res 33:253-264 (2021). PubMed: 34603794
- Serafim RAM et al. Discovery of a Potent Dual SLK/STK10 Inhibitor Based on a Maleimide Scaffold. J Med Chem 64:13259-13278 (2021). PubMed: 34463505
- Qin Y et al. Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. Sci Adv 6:eaaw9960 (2020). PubMed: 32128390
- Chen L et al. Advanced glycation end products induce immature angiogenesis in in vivo and ex vivo mouse models. Am J Physiol Heart Circ Physiol 318:H519-H533 (2020). PubMed: 31922896
- Hu S et al. CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin. Cancer Cell Int 20:563 (2020). PubMed: 33292278
- Chen W et al. Role of TLR4-MAP4K4 signaling pathway in models of oxygen-induced retinopathy. FASEB J N/A:fj201801086RR (2018). PubMed: 30475644
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978