重组Anti-MMS22L (C6ORF167)抗体[EPR13654] (ab181047)
![Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.cn/ps/products/181/ab181047/Images/ab181047-478712-anti-mms22l-c6orf167-antibody-epr13654-westernblot-human-mouse-rat.jpg "Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167)
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MMS22L (C6ORF167)抗体[EPR13654]
参阅全部 MMS22L (C6ORF167) 一抗 -
描述
兔单克隆抗体[EPR13654] to MMS22L (C6ORF167) -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13654 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181047于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
|
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| IP |
1/20.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa). |
|
IP
1/20. |
靶标
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功能
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. -
序列相似性
Belongs to the MMS22 family. MMS22L subfamily. -
细胞定位
Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks. - Information by UniProt
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数据库链接
- Entrez Gene: 253714 Human
- Entrez Gene: 212377 Mouse
- Entrez Gene: 313108 Rat
- Omim: 615614 Human
- SwissProt: Q6ZRQ5 Human
- SwissProt: B1AUR6 Mouse
- Unigene: 444292 Human
- Unigene: 120919 Mouse
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别名
- C6orf167 antibody
- C6orf167 chromosome 6 open reading frame 167 antibody
- chromosome 6 open reading frame 167 antibody
see all
图片
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Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : LLC1 (Mouse lung carcinoma ) whole cell lysate
Lane 4 : Mouse testis lysate
Lane 5 : Mouse spleen lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 142 kDa -
![Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.cn/ps/products/181/ab181047/Images/ab181047-7-anti-mms22l-c6orf167-antibody-epr13654-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Purified ab181047 at 1:20 dilution (0.5µg) immunoprecipitating MMS22L (C6ORF167) in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg.
Lane 2 (+): ab181047 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181047 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDa -
![Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.cn/ps/products/181/ab181047/Images/ab181047-6-anti-mms22l-c6orf167-antibody-epr13654-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MMS22L (C6ORF167) with Purified ab181047 at 1:20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.cn/ps/products/181/ab181047/Images/ab181047-4-benefits-of-recombinant-antibodies.png)
Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab181047 被引用在 5 文献中.
- Zhang J et al. Symmetric control of sister chromatid cohesion establishment. Nucleic Acids Res 51:4760-4773 (2023). PubMed: 36912084
- Guo Z et al. Integrative pan-cancer landscape of MMS22L and its potential role in hepatocellular carcinoma. Front Genet 13:1025970 (2022). PubMed: 36276962
- Luo Q et al. MMS22L Expression as a Predictive Biomarker for the Efficacy of Neoadjuvant Chemoradiotherapy in Oesophageal Squamous Cell Carcinoma. Front Oncol 11:711642 (2021). PubMed: 34660277
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807