AB188156

Anti-MMS19抗体

Name: MMS19抗体 (ab188156)| Abcam中文官网
Brand: Abcam Limited
SKU: AB188156
Price: 5757 CNY
Availability: InStock

Anti-MMS19 antibody

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(2 Publications)

Rabbit Polyclonal MMS19 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MMS19 aa 650-800.

查看别名

MMS19L, MMS19, MMS19 nucleotide excision repair protein homolog, hMMS19, MET18 homolog, MMS19-like protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

Paraffin embedded human testis tissue stained for MMS19 using ab188156 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

Caco-2 cells stained for MMS19 (green) using ab188156 at 2 μg/ml in ICC/IF.

Supplier Data

Western blot - Anti-MMS19 antibody (AB188156)

All lanes:

Western blot - Anti-MMS19 antibody (ab188156) at 1/250 dilution

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RT-4 cell lysate

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U-251 MG sp cell lysate

Predicted band size: 113 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MMS19 aa 650-800. The exact immunogen used to generate this antibody is proprietary information.

Q96T76

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein MMS19 also known as MMS19L or mitotic spindle assembly 19 plays an important role in DNA repair and ribonucleotide reduction. It is a component of the cytoplasmic iron-sulfur protein assembly (CIA) machinery. MMS19 is approximately 104 kDa in mass and is expressed in a variety of tissues with higher expression noted in testis and thymus. The protein has been observed in the nucleus and cytoplasm where it participates in DNA-related and metabolic processes.

Biological function summary

MMS19 functions as a core component of the cytoplasmic iron-sulfur protein assembly (CIA) targeting complex. This complex targets specific iron-sulfur cluster-containing proteins which are essential for DNA repair and other cellular processes. MMS19 facilitates the incorporation of iron-sulfur clusters into target proteins which is critical for their function. It interacts with other proteins like CIAO1 and FAM96B within the complex indicating its collaborative role in cellular maintenance and genomic stability.

Pathways

MMS19 is actively engaged in the DNA repair and replication pathways. It plays a role in nucleotide excision repair (NER) and is important in maintaining genomic integrity through its involvement in DNA synthesis and repair. The protein associates with helicase-like transcription factor (HLTF) and other related proteins within these pathways helping coordinate the response to DNA damage and repair mechanisms.

MMS19's dysfunction has been linked to various cancer types due to its central role in DNA repair. Defects in MMS19 can lead to genomic instability a hallmark of cancerous transformations. Moreover its interaction with other proteins in the repair pathways such as ERCC2 links it to disorders like xeroderma pigmentosum. This connection suggests its importance in protecting against DNA damage and maintaining genomic stability.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity (PubMed : 29848660). In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed : 22678361, PubMed : 22678362, PubMed : 23585563, PubMed : 29225034). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD (PubMed : 20797633). Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis (PubMed : 29848660). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed : 11279242).

See full target information MMS19

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 292:13879-13889 PubMed28615450

2017

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.

Applications

Unspecified application

Species

Unspecified reactive species

Arunkumar S Upadhyay,Oliver Stehling,Christakis Panayiotou,Ralf Rösser,Roland Lill,Anna K Överby

DNA repair 43:9-17 PubMed27235625

2016

Human DNA polymerase ε is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana N Moiseeva,Armin M Gamper,Brian L Hood,Thomas P Conrads,Christopher J Bakkenist

View all publications

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Anti-MMS19抗体

Anti-MMS19 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS19 antibody (AB188156)

Immunocytochemistry/ Immunofluorescence - Anti-MMS19 antibody (AB188156)

Western blot - Anti-MMS19 antibody (AB188156)