重组Anti-MMP8抗体[EP1252Y] (ab81286)
![Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/ab81286-541946-anti-mmp8-antibody-pP1252y-western-blot-mouse-lung.jpg "Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1252Y] to MMP8
- Suitable for: WB, IHC-P, IP
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP8抗体[EP1252Y]
参阅全部 MMP8 一抗 -
描述
兔单克隆抗体[EP1252Y] to MMP8 -
宿主
Rabbit -
特异性
WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow. Not suitable in IHC-P for Mouse samples.
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经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Rat lung lysate, mouse spleen and mouse lung tissue IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1252Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
1/50.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 53 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
1/50. |
靶标
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功能
Can degrade fibrillar type I, II, and III collagens. -
组织特异性
Neutrophils. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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数据库链接
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Entrez Gene: 63849 Rat
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- SwissProt: O88766 Rat
- Unigene: 161839 Human
see all -
别名
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
图片
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Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsDilution and blocking buffer: 5% NFDM/TBST
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
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![Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/ab81286-541945-anti-mmp8-antibody-pP1252y-western-blot-mouse-brain.jpg)
Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)All lanes : Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsDilution and blocking buffer: 5% NFDM/TBST
Negative control: mouse brain.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
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![Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-11.jpg)
Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution + Rat lung lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-13.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/ab81286-4-anti-mmp8-antibody-ep1252y-immunoprecipitation-lung-rat.jpg)
Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10µg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.cn/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-15.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab81286 被引用在 39 文献中.
- Nguyen BT et al. Melatonin inhibits chondrosarcoma cell proliferation and metastasis by enhancing miR-520f-3p production and suppressing MMP7 expression. J Pineal Res 75:e12872 (2023). PubMed: 37057370
- Zhang JB et al. Therapeutic Effect of Prolyl Endopeptidase Inhibitor in High-fat Diet-induced Metabolic Dysfunction-associated Fatty Liver Disease. J Clin Transl Hepatol 11:1035-1049 (2023). PubMed: 37577240
- Wu W et al. Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC. Int J Biol Sci 19:2848-2859 (2023). PubMed: 37324952
- Jiang Y et al. LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35884594
- Kirketerp-Møller K et al. Biomarkers of Skin Graft Healing in Venous Leg Ulcers. Acta Derm Venereol 102:adv00749 (2022). PubMed: 35604238