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重组RabMAb

重组Anti-MMP7抗体[EPR1251(2)] (ab176325)

Submit a review Submit a question References (3)
Western blot - Anti-MMP7 antibody [EPR1251(2)] (ab176325)
  • Flow Cytometry (Intracellular) - Anti-MMP7 antibody [EPR1251(2)] (ab176325)
  • Anti-MMP7 antibody [EPR1251(2)] (ab176325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1251(2)] to MMP7
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MMP7抗体[EPR1251(2)]
    参阅全部 MMP7 一抗

  • 描述

    兔单克隆抗体[EPR1251(2)] to MMP7

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human prostate, BxPC-3 and Human colon cancer lysates. BxPC-3 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176325于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

  • 序列相似性

    Belongs to the peptidase M10A family.

  • 结构域

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    图片

    • Western blot - Anti-MMP7 antibody [EPR1251(2)] (ab176325)
      Western blot - Anti-MMP7 antibody [EPR1251(2)] (ab176325)
      All lanes : Anti-MMP7 antibody [EPR1251(2)] (ab176325) at 1/1000 dilution

      Lane 1 : Human prostate lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Flow Cytometry (Intracellular) - Anti-MMP7 antibody [EPR1251(2)] (ab176325)
      Flow Cytometry (Intracellular) - Anti-MMP7 antibody [EPR1251(2)] (ab176325)

      Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labeling MMP7using ab176325 at a 1/100 dilution (red) or a rabbit IgGcontrol (green). 

       

       

    • Anti-MMP7 antibody [EPR1251(2)] (ab176325)
      Anti-MMP7 antibody [EPR1251(2)] (ab176325)

    文献 (3)

    发表研究结果有使用 ab176325？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab176325 被引用在 3 文献中.

    • Kumar P  et al. The matrix metalloproteinase 7 (MMP7) links Hsp90 chaperone with acquired drug resistance and tumor metastasis. Cancer Rep (Hoboken) N/A:e1261 (2020). PubMed: 32761892
    • Li X  et al. Advanced Oxidation Protein Products Promote Lipotoxicity and Tubulointerstitial Fibrosis via CD36/ß-Catenin Pathway in Diabetic Nephropathy. Antioxid Redox Signal 31:521-538 (2019). PubMed: 31084358
    • Xie Y  et al. MMP7 interacts with ARF in nucleus to potentiate tumor microenvironments for prostate cancer progression in vivo. Oncotarget 7:47609-47619 (2016). WB . PubMed: 27356744

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