Anti-MMP3抗体(ab223666)
Key features and details
- Goat polyclonal to MMP3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MMP3抗体
参阅全部 MMP3 一抗 -
描述
山羊多克隆抗体to MMP3 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Dog
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免疫原
Synthetic peptide corresponding to Human MMP3 aa 450 to the C-terminus (C terminal) (Cysteine residue). NP_002413.1
Database link: P08254 -
阳性对照
- WB: Mouse eye lysate; human prostate lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 403445 Dog
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 100009111 Rabbit
- Omim: 185250 Human
- SwissProt: Q6Y4Q5 Dog
- SwissProt: Q28397 Horse
- SwissProt: P08254 Human
see all -
别名
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
图片
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Western blot - Anti-MMP3 antibody (ab223666)Anti-MMP3 antibody (ab223666) at 0.3 µg/ml + mouse eye lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour
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Western blot - Anti-MMP3 antibody (ab223666)Anti-MMP3 antibody (ab223666) at 0.2 µg/ml + human prostate lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour
数据表及文件
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Datasheet download
文献 (1)
ab223666 被引用在 1 文献中.
- Guo S et al. Single-cell RNA-seq analysis reveals that immune cells induce human nucleus pulposus ossification and degeneration. Front Immunol 14:1224627 (2023). PubMed: 37638033
