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AB97779

MMP2抗体

Anti-MMP2 antibody

4

(6 Reviews)

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(258 Publications)

Anti-MMP2 antibody (ab97779) is a rabbit polyclonal antibody detecting MMP2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Over 190 publications
- Trusted since 2010

查看别名

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB97779)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MMP2 antibody (AB97779)

Immunofluorescence analysis of 4% paraformaldehyde fixed U87-MG cells labelling MMP2 protein at cytoplasm using ab97779 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker was stained with anti-alpha Tubulin antibody at a 1/1000 dilution (Red). The nuclear counter stain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human lung cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human colon cancer tissue stained for MMP2 protein at extracellular matrix with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

Paraffin-embedded human breast carcinoma tissue stained for MMP2 protein at cytosol and nucleus with ab97779 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation - Anti-MMP2 antibody (AB97779)
  • IP

Supplier Data

Immunoprecipitation - Anti-MMP2 antibody (AB97779)

MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 μg ab97779. Western blot was performed from the immunoprecipitate using ab97779.

Lane 1 : Control IgG IP in U-87 MG whole cell extracts.

Lane 2 : ab97779 IP in U-87 MG whole cell extracts.

All lanes:

Immunoprecipitation - Anti-MMP2 antibody (ab97779)

Predicted band size: 73 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

WB, IP, ICC/IF, IHC-P

applications

免疫原

Synthetic Peptide within Human MMP2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08253

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替代产品

Primary Antibodies

AB92536

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Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

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Primary Antibodies

AB218462

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Primary Antibodies

AB2462

Anti-MMP2 antibody [4D3]

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Primary Antibodies

AB86607

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (AB86607)

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Primary Antibodies

AB264081

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] - BSA and Azide free (AB264081)

  • 2
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Primary Antibodies

AB181286

Anti-MMP2 antibody [EPR17003-25]

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  • 0
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Primary Antibodies

AB215986

Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free

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Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (AB215986)

  • 0
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搭配使用产品

Primary Antibodies

AB283575

Anti-MMP9 antibody [RM1020]

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-MMP2 antibody (ab97779) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of MMP2?
Anti-MMP2 (ab97779) specifically detects a band for MMP2 (UniProt: P08253) at a molecular weight of 74kDa.

Trusted by the scientific community
Anti-MMP2 (ab97779) was first used in a scientific publication in 2010 and has been cited over 190 times in peer-reviewed journals.

Reviewed by scientists
Anti-MMP2 (ab97779) has over 5 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information MMP2
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文献 (258)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40943413

2025

β-Caryophyllene Ameliorates Thioacetamide-Induced Liver Fibrosis in Rats: A Preventative Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Lujain Bader Eddin,Amar Mahgoub,Saeeda Almarzooqi,Ernest Adeghate,Sandeep B Subramanya,Shreesh Ojha

Gut microbes 17:2534677 PubMed40749709

2025

Harnessing EP21 and its membrane vesicles to inhibit myopia development.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Fong Lin,Yu-An Hsu,Yung-Lan Chou,Ying-Chi Chen,En-Shyh Lin,Peng-Tai Tien,Jamie Jiin-Yi Chen,Ming-Yen Wu,Chia-Hung Lin,Hui-Ju Lin,Lei Wan

Obstetrics & gynecology science 68:408-417 PubMed40610038

2025

Correlation of VEGF, HIF-1α, and MMP2 expression in placental villi among patients with recurrent spontaneous abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Ju,Xiaolin Hou,Yage Wang,Xiaofeng Zhang,Yan Feng

Cancer medicine 14:e71013 PubMed40583724

2025

MMRN1 Facilitates Renal Cell Carcinoma by Activating AMPK/MMPs Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingji Ye,Jian Cao,Zhihao Ming,Yu Xie

Chemical biology & drug design 105:e70145 PubMed40527582

2025

Exploring Flavonoids as Regulators of MMP-2 and MMP-9 in Cancer Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Vy Thi Vo,Geewoo Nam Patton,Song Ja Kim,Namdoo Kim,Hyuck Jin Lee

Frontiers in pharmacology 16:1575635 PubMed40520168

2025

Biodegradable sustained-release microneedle patch loaded with clindamycin hydrochloride: a breakthrough in acne management.

Applications

Unspecified application

Species

Unspecified reactive species

Haomei Fan,Ruohan Liao,Yiling Yang,Yan Xing,Chengdong Zhang,Xuwei Luo,Chao Pu,Liling Wu,Xingping Li,Juhua Zhao,Dongqin Xiao

Oncology research 33:1473-1484 PubMed40486873

2025

Gallic acid suppresses esophageal squamous cell carcinoma progression and enhances cisplatin chemosensitivity through IL-6/STAT3/Notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuran Bedolla,Hao Wu,Linyu Liu,Xueting Liu,Yanli Ren

BMC musculoskeletal disorders 26:538 PubMed40450237

2025

Effect of intraarticular human umbilical cord mesenchymal stem cells transplantation on cartilage degradation and matrix metalloproteinases in OA rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqun Li,Qipeng Han,Shayan Chen,Wenlong Huang,Yanqing Chen,Zhihuang Wu,Fengming Tan,Muyun Liu,Jiangying Zou

Cancer medicine 14:e70701 PubMed40062588

2025

The Role of Chemokine (C-C Motif) Ligand 7 (CCL7) in Hepatocellular Carcinoma: Expression, Function, and Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yangkun Luo,Fei Wan,Zhao Zhang,Zujun Qin,Yongjun Ren

Molecular medicine reports 31: PubMed39981899

2025

Recombinant human collagen XVII protects skin basement membrane integrity by inhibiting the MAPK and Wnt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Simin Lin,Yun Wei,Zhangying Ye
View all publications
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