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AB264081

Anti-MMP2抗体[6E3F8] - BSA and Azide free

Anti-MMP2 antibody [6E3F8] - BSA and Azide free

2

(1 Review)

|

(1 Publication)

Mouse Monoclonal MMP2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] - BSA and Azide free (AB264081)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] - BSA and Azide free (AB264081)

Immunohistochemistry of human pancreas carcinoma staining MMP2 with ab86607 at 1μg/ml.

This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab86607).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] - BSA and Azide free (AB264081)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] - BSA and Azide free (AB264081)

IHC image of ab86607 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86607, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab86607).

不同偶联物与剂型 (1)

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

6E3F8

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB97779

Anti-MMP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody (AB97779)

  • 4
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Primary Antibodies

AB218462

Anti-MMP2 antibody [MMP2/1501]

Protein Array - Anti-MMP2 antibody [MMP2/1501] (AB218462)

  • 0
  • 0
Primary Antibodies

AB2462

Anti-MMP2 antibody [4D3]

Western blot - Anti-MMP2 antibody [4D3] (AB2462)

  • 4
  • 3
Primary Antibodies

AB237473

Alexa Fluor® 488 Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MMP2 antibody [EPR1184] (AB237473)

  • 0
  • 0
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB181286

Anti-MMP2 antibody [EPR17003-25]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] (AB181286)

  • 0
  • 0
Primary Antibodies

AB250476

Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (AB250476)

  • 0
  • 0
Primary Antibodies

AB215986

Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MMP2 antibody [EPR1184] - Low endotoxin, Azide free (AB215986)

  • 0
  • 0
Primary Antibodies

AB237474

Alexa Fluor® 647 Anti-MMP2 antibody [EPR1184]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MMP2 antibody [EPR1184] (AB237474)

  • 0
  • 0

搭配使用产品

Assay Kits

AB139446

MMP2 Inhibitor Screening Assay Kit (Colorimetric)

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

  • 0
  • 0
Primary Antibodies

AB283575

Anti-MMP9 antibody [RM1020]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-MMP9 antibody [RM1020] (AB283575)

  • 0
  • 0
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB51074

Anti-MMP14 antibody [EP1264Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-MMP14 antibody [EP1264Y] (AB51074)

  • 4
  • 20
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

ab264081 is the carrier-free version of ab86607.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information MMP2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou
View all publications
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Abcam Product Promise

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