Anti-MMP19抗体(ab53146)
Key features and details
- Rabbit polyclonal to MMP19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MMP19抗体
参阅全部 MMP19 一抗 -
描述
兔多克隆抗体to MMP19 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide derived from human MMP19.
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阳性对照
- WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53146 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53146于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin. -
组织特异性
Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients. -
疾病相关
May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Activated by autolytic cleavage after Lys-97. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4327 Human
- Entrez Gene: 304608 Rat
- Omim: 601807 Human
- SwissProt: Q99542 Human
- Unigene: 591033 Human
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别名
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
- Matrix metalloproteinase RASI antibody
see all
图片
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Western blot - Anti-MMP19 antibody (ab53146)All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution
Lane 1 : Extracts of Huv Ec cells with no peptide
Lane 2 : Extracts of Huv Ec cells with peptide
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP19 antibody (ab53146)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab53146 被引用在 8 文献中.
- Li D et al. MicroRNA 211-5p inhibits cancer cell proliferation and migration in pancreatic cancer by targeting BMP2. Aging (Albany NY) 15:14411-14421 (2023). PubMed: 38059889
- Xu L et al. Identification of macrophage correlated biomarkers to predict the prognosis in patients with intrahepatic cholangiocarcinoma. Front Oncol 12:967982 (2022). PubMed: 36158683
- Zhang J et al. Long non-coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA-324-5p and upregulating KCTD20 expression. Oncol Rep 46:N/A (2021). PubMed: 33982764
- Tian G et al. MicroRNA-16 inhibits the proliferation, migration and invasion of non-small cell lung carcinoma cells by down-regulating matrix metalloproteinase-19 expression. Eur Rev Med Pharmacol Sci 23:5260-5269 (2019). PubMed: 31298377
- Shen ZG et al. Knockdown of E2F3 Inhibits Proliferation, Migration, and Invasion and Increases Apoptosis in Glioma Cells. Oncol Res 25:1555-1566 (2017). PubMed: 28337965
- Liu RY et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . PubMed: 23956056
- Wang L et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). PubMed: 22855183
- Jackson V et al. Matrix metalloproteinase 14 and 19 expression is associated with thoracic aortic aneurysms. J Thorac Cardiovasc Surg 144:459-66 (2012). Human . PubMed: 21955474