Anti-MMP16抗体(ab73877)
Key features and details
- Rabbit polyclonal to MMP16
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MMP16抗体
参阅全部 MMP16 一抗 -
描述
兔多克隆抗体to MMP16 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Rat, Human -
免疫原
A synthetic peptide mapping at the C-terminal of human MMP16.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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| IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
|
| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. -
组织特异性
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
发展阶段
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells. - Information by UniProt
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数据库链接
- Entrez Gene: 4325 Human
- Entrez Gene: 65205 Rat
- Omim: 602262 Human
- SwissProt: P51512 Human
- SwissProt: O35548 Rat
- Unigene: 492187 Human
- Unigene: 546267 Human
- Unigene: 208361 Rat
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别名
- C8orf57 antibody
- DKFZp761D112 antibody
- Matrix metalloproteinase 16 antibody
see all
图片
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Western blot - Anti-MMP16 antibody (ab73877)All lanes : Anti-MMP16 antibody (ab73877) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human Hela cells.
Lane 2 : Whole cell lysates prepared from Human Jurkat cells.
Lane 3 : Whole cell lysates prepared from Human Colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP16 antibody (ab73877)Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml. -
Immunohistochemistry (Frozen sections) - Anti-MMP16 antibody (ab73877)Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab73877 被引用在 15 文献中.
- Wu W et al. Liver X receptors and estrogen receptor β, two players in a rare subtype of NSCLC. Int J Biol Sci 19:2848-2859 (2023). PubMed: 37324952
- Xu C et al. ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/ß-Catenin Signaling. Mol Cancer Res 19:1441-1453 (2021). PubMed: 34158395
- Schniers BK et al. PEPT1 is essential for the growth of pancreatic cancer cells: a viable drug target. Biochem J 478:3757-3774 (2021). PubMed: 34569600
- Zhou H & Chen Y CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway. Braz J Med Biol Res 54:e11459 (2021). PubMed: 34730679
- Zhang Y et al. Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs. Bioengineered 12:12767-12777 (2021). PubMed: 34747300