Anti-MMP14抗体(ab53712)
Key features and details
- Rabbit polyclonal to MMP14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MMP14抗体
参阅全部 MMP14 一抗 -
描述
兔多克隆抗体to MMP14 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MMP14 aa 471-520 (C terminal).
Database link: P50281 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53712于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
|
| IHC-P | (1) |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
|
IHC-P
1/50 - 1/100. |
靶标
-
功能
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. -
组织特异性
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 4323 Human
- Omim: 600754 Human
- SwissProt: P50281 Human
- Unigene: 2399 Human
-
别名
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
see all
图片
-
Western blot - Anti-MMP14 antibody (ab53712)All lanes : Anti-MMP14 antibody (ab53712) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab53712)ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody (ab53712)ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.
数据表及文件
-
SDS download
-
Datasheet download
文献 (33)
ab53712 被引用在 33 文献中.
- Zhu L et al. Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption. J Cell Biol 222:N/A (2023). PubMed: 36880731
- Oezel L et al. Dupuytren's Disease Is Mediated by Insufficient TGF-β1 Release and Degradation. Int J Mol Sci 24:N/A (2023). PubMed: 37894778
- Raute K et al. Breast Cancer Stem Cell-Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands. Cancer Immunol Res 11:810-829 (2023). PubMed: 37139603
- Tang Y et al. Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate. Dev Cell 57:480-495.e6 (2022). PubMed: 35150612
- Thakur V et al. Targeting extracellular matrix remodeling sensitizes glioblastoma to ionizing radiation. Neurooncol Adv 4:vdac147 (2022). PubMed: 36212741