Anti-MMP13抗体(ab84594)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MMP13抗体
参阅全部 MMP13 一抗 -
描述
兔多克隆抗体to MMP13 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse
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免疫原
Synthetic peptide corresponding to Human MMP13 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93499) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa). |
靶标
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功能
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
组织特异性
Seems to be specific to breast carcinomas. -
疾病相关
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Entrez Gene: 171052 Rat
- Omim: 600108 Human
- SwissProt: O18927 Horse
- SwissProt: P45452 Human
- SwissProt: P33435 Mouse
- SwissProt: O62806 Rabbit
see all -
别名
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
图片
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Western blot - Anti-MMP13 antibody (ab84594)All lanes : Anti-MMP13 antibody (ab84594) at 1 µg/ml
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The bands observed at 46 kDa could potentially be a cleaved form of Human Collagenase 3 precursor. The first 19 amino acids in the sequence act as a signal peptide and the following 84 (A.A 20-103) act as a activation peptide. Once cleaved this gives the target protein an expected weight of 43 kDa.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab84594 被引用在 26 文献中.
- Li G et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J Orthop Translat 38:241-255 (2023). PubMed: 36514714
- Supolikova M et al. Murine herpesvirus-68-related growth factors treatment correlates with decrease of p53 and HIF-1α protein levels. Pathog Dis 81:N/A (2023). PubMed: 36997335
- Zhu Y et al. MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritis. J Orthop Surg Res 18:186 (2023). PubMed: 36894989
- Jiang H et al. Innate/Inflammatory Bioregulation of Surfactant Protein D Alleviates Rat Osteoarthritis by Inhibiting Toll-Like Receptor 4 Signaling. Front Immunol 13:913901 (2022). PubMed: 35865531
- Yan J et al. Selonsertib Alleviates the Progression of Rat Osteoarthritis: An in vitro and in vivo Study. Front Pharmacol 12:687033 (2021). PubMed: 34322020