Anti-MMP11抗体[OTI3E10] - BSA and Azide free (ab273673)
![Western blot - Anti-MMP11 antibody [OTI3E10] - BSA and Azide free (ab273673)](https://www.abcam.cn/ps/products/273/ab273673/Images/ab273673-394424-AntiMMP11antibodyOTI3E10westernblotMDAMB231T47Dhuman.jpg "Western blot - Anti-MMP11 antibody [OTI3E10] - BSA and Azide free (ab273673)")
Key features and details
- Mouse monoclonal [OTI3E10] to MMP11 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-MMP11抗体[OTI3E10] - BSA and Azide free
参阅全部 MMP11 一抗 -
描述
小鼠单克隆抗体[OTI3E10] to MMP11 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MMP11 aa 200 to the C-terminus.
Database link: P24347 -
阳性对照
- WB: MDA-MB-231 and T-47D cell lysate. pCMV6-ENTRY MMP11 cDNA transfected HEK-293T cell lysate.
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常规说明
ab273673 is the carrier free version of ab236510.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
OTI3E10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273673于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 55 kDa.
|
| 说明 |
|---|
|
WB
1/500. Predicted molecular weight: 55 kDa. |
靶标
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功能
May play an important role in the progression of epithelial malignancies. -
组织特异性
Specifically expressed in stromal cells of breast carcinomas. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4320 Human
- Omim: 185261 Human
- SwissProt: P24347 Human
- Unigene: 143751 Human
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别名
- Matrix Metalloproteinase 11 antibody
- Matrix metalloproteinase-11 antibody
- MMP-11 antibody
see all
图片
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Western blot - Anti-MMP11 antibody [OTI3E10] - BSA and Azide free (ab273673)All lanes : Anti-MMP11 antibody [OTI3E10] (ab236510) at 1/500 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 55 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236510).
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![Western blot - Anti-MMP11 antibody [OTI3E10] - BSA and Azide free (ab273673)](https://www.abcam.cn/ps/products/273/ab273673/Images/ab273673-394423-AntiMMP11antibodyOTI3E10westernblottransfectedHEK293T.jpg)
Western blot - Anti-MMP11 antibody [OTI3E10] - BSA and Azide free (ab273673)All lanes : Anti-MMP11 antibody [OTI3E10] (ab236510) at 1/500 dilution
Lane 1 : HEK-293T cells transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY MMP11 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236510).
Cells were transfected for 48 hours then lysed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab273673 尚未被引用在任何文献中。
